The comprehensive reconstruction of cell lineages in complex multicellular organisms is a central goal of developmental biology. We present an open-source computational framework for segmentation and tracking of cell nuclei with high accuracy and speed. We demonstrate its (1) generality, by reconstructing cell lineages in four-dimensional, terabyte-sized image data of fruit-fly, zebrafish and m...

The following summary tries to capture a collection of state-of-the-art techniques and challenges for future work on lineage management in uncertain and probabilistic databases that we discussed in our working group. It was one half of a larger committee that we had initially formed, which then got split into two groups—one focusing on lineage as a means of explanation of data, and one focusing...

Data lineage plays a critical role in verifying data correctness in scientific databases and data warehousing. In this paper, we clearly define forward data lineage in bag semantics and show its properties. We propose a tracing method that piggybacks normal query evaluation. Our method effectively supports aggregation and variable granularity lineage. More importantly, it features cost effectiv...

Multiple sclerosis (MS) is an autoimmune disease which affects myelin in the central nervous system (CNS) and leads to serious disability. Currently available treatments for MS mainly suppress the immune system. Regenerative medicine-based approaches attempt to increase myelin repair by targeting endogenous progenitors or transplanting stem cells or their derivatives. Fingolimod exerts anti-inf...

moulting and is unable to attack. However, if a male encounters a mature female, he may court her and if the female accepts, the male inserts the first palp. He then has to withdraw, re-court and insert the second palp. But in this situation the female can attack at any time. The researchers therefore looked at the fate of males under both mating strategies in natural populations, in caged stud...

Discussion of the lineage and transformation of esophageal epithelium as they relate to gastroesophageal reflux disease (GERD) requires consideration of both the native squamous epithelium and the acquired columnar epithelium of Barrett's esophagus. The non-keratinizing squamous epithelium has a proliferative basal zone where cells undergo mitosis, then migrate toward the luminal surface, and d...

Listeria monocytogenes is a serious food-borne pathogen that can cause invasive disease in humans and other animals and has been the leading cause of food recalls due to microbiological concerns in recent years. In order to test hypotheses regarding L. monocytogenes lineage composition, evolution, ecology, and taxonomy, a robust intraspecific phylogeny was developed based on prfA virulence gene...

The study included 1742 patients with acute myeloblastic leukaemias (AML) and acute lymphoblastic leukaemias (ALL), Kyiv city residents and patients from 20 regions of Ukraine. Bone marrow and blood smears were sent at diagnosis to Reference Center. The analysis was based on May-Grünvald-Giemza (MGG) stain and cytochemical reactions (MPO, acNSE, CAE, AP, PAS). Immunocytochemical techniques (APA...

The cell lineage tree of a multicellular organism represents its history of cell divisions from the very first cell, the zygote. A new method for high-resolution reconstruction of parts of such cell lineage trees was recently developed based on phylogenetic analysis of somatic mutations accumulated during normal development of an organism. In this study we apply this method in mice to reconstru...