An extra cellular lipase was isolated and purified from the culture broth of Pseudomonas aeruginosa SRT 9 to apparent homogeneity using ammonium sulfate precipitation followed by chromatographic techniques on phenyl Sepharose CL- 4B and Mono Q HR 5/5 column, resulting in a purification factor of 98 fold with specific activity of 12307.8 U/mg. The molecular weight of the purified lipase was esti...

1. Isolated cardiac-muscle cells from the hearts of adult rats were shown to retain a high amount of viability during 4h of incubation when viability was assessed by Trypan Blue stain exclusion and intracellular enzyme leakage. 2. The cells also retained their ability to take up °2 and utilize added substrates over the period of incubation at both 25 and 300C. 3. When cells from the hearts of f...

OBJECTIVES To assess the role of serum amylase and lipase in the diagnosis of acute pancreatitis. Secondary aims were to perform a cost analysis of these enzyme assays in patients admitted to the surgical admissions unit. DESIGN Cohort study. SETTING Secondary care. PARTICIPANTS Patients admitted with pancreatitis to the acute surgical admissions unit from January to December 2010 were in...

Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between th...

BACKGROUND AND OBJECTIVES Lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. The aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. MATERIALS AND METHODS A number of bacteria were isolated from sites related to the oil industries. An isolat...

An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum temperature and pH for the purified lip...

Enantioselective resolution of a racemate cis-3 is performed by Candida antarctica lipase B. The hydrolyzed product in its (2R,3S) can be used for the preparation neuromediator analogue, while non-reacted (2S,3R) substrate building block antibiotic Moxifloxacin. enzyme shows both enantioselectivity as well regioselectivity ester position 3.

The biofilm degradation of Aggregatibacter actinomycetemcomitans is essential as a complete periodontal disease therapy, and here we show the effects of potential probiotic bacteria such as Lactobacillus spp. for the biofilm of several serotypes of A. actinomycetemcomitans strains. Eight of the 13 species showed the competent biofilm degradation of ≥ 90% reduction in biofilm values in A. actino...

Hormone-sensitive lipase from rat adipose tissue was solubilized with nonionic detergent and purified 2,000fold to 50% protein purity by repeated gradient sievorptive chromatography on diethyL(2-hydroxypropy1)aminoethyl-Sephadex and by affinity chromatography on triacylglycerol-containing polyacrylamideagarose gel. The identity of the enzyme protein, a polypeptide with M, = 84,000 by polyacryla...