Nicotine, a pharmacologically active substance in tobacco, has been identified as a major risk factor for lung diseases. In the present study, we evaluated the protective effects of curcumin on tissue lipid peroxidation and antioxidants in nicotine-treated Wistar rats. Lung toxicity was induced by subcutaneous injection of nicotine at a dose of 2.5 mg/kg (5 days a week, for 22 weeks). Curcumin ...

The influence of soy-protein diet on brain lipid peroxidation in female rats was studied using a tail-suspension model of weightlessness. The study tested the efficacy of diets containing 0% or 11.1% soy-protein in 4 groups of female Sprague Dawley rats that were maintained with or without tail-suspension for a period of 3 weeks. At term, the whole brain was removed, segmented, and analyzed for...

OBJECTIVE To investigate the effects of fluoride on lipid peroxidation, DNA damage and apoptosis in human embryo hepatocyte L-02 cells. METHODS Lipid peroxide (LPO) level, reduced glutathione (GSH) content, DNA damage, apoptosis, and cell cycle analysis were measured after in vitro cultured L-02 cells were exposed to sodium fluoride at different doses (40 microg/mL, 80 microg/mL, and 160 micr...

Increased oxidative stress is the consequence of either enhanced reactive oxygen species (ROS) production or attenuated ROS scavenging capacity, resulting in tissue damage that in most instances is assessed by the measurement of lipid peroxides. In the current study, diabetes-induced lipid peroxidation in rat liver microsomal membranes was investigated by Fourier transform infrared (FT-IR) spec...

A study in a recent issue of the highly respected journal Science reported that lipid hydroperoxides (rancid fat molecules) react with vitamin C to form products that could potentially harm DNA. Although the reaction of these products with DNA was not demonstrated in the study, the authors suggested that vitamin C can form genotoxins (DNA-damaging agents) from lipid hydro-peroxides, titling the...

Background. The Free Radical Theory of Aging mechanistically links oxidative stress to aging. Okinawa has among the world's longest-lived populations but oxidative stress in this population has not been well characterized. Methods. We compared plasma lipid peroxide (LPO) and vitamin E-plasma and intracellular tocopherol levels (total α, β, and γ), in centenarians with younger controls. Results....

I . A micro-adaptation of the iodimetric method has been used to determine lipid peroxides in the tissues of vitamin E-deficient rats and chicks. 2. No increases in lipid peroxide were found in liver, kidney or adipose tissue of rats with nutritional liver necrosis due to deficiency of vitamin E and selenium. When liver necrosis was induced by giving rats a casein diet and silver acetate soluti...