Among the numerous anti-sperm monoclonal antibodies generated in our laboratory, HS-33 was shown to react with a conserved antigen on the acrosome of spermatozoa from human and mouse. By using indirect immunofluorescent assay, it was demonstrated that HS-33 did not bind to live human sperm. However, this antibody was found to react with the methanol-fixed acrosome-intact, but not with acrosome-...

Motility parameters of the spermatozoa in most fish species spawning in fresh water like Rutilus kutum lasts for a short time after activation. Ovarian fluid significantly influenced sperm motility (motility duration period) and percent motility (progressive forward motile sperm). Both of these variables generally increased as the concentration of ovarian fluid increased from 33% to 50%, respec...

objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...

Background: Semen quality is affected by environmental and genetically factors. The present study aimed to determine the association of varicocele surgery and males' infertility based on semen analysis. Materials and Methods: In this case-control study, 188 men with infertility disorder in Ilam province were enrolled between May 2008 and May 2009. Semen analysis was done using the Weili Dy...

PURPOSE We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. MATERIALS AND METHODS Indications for cryopreservation include several applications, namely homologo...

This article presents an update on the variable prognostic significance of different sperm pathologies in patients with severe male factor infertility due to morphology and motility disorders. Severe asthenozoospermia is one of the leading causes of male infertility as spermatozoa cannot reach the oocyte and/or penetrate normally. Identifying structural causes of sperm immotility was of great c...

This study investigates the effect of supplementing bovine follicular fluids (FF) to rabbit sperm motility, acrosome reaction and transaminases activities. Ovarian FF were collected from 46 pairs of bovine ovaries. Normal follicles were classified, according to their diameters, into small (SFF, ≤4 mm), medium (MFF,>4-8 mm), large (LFF,> 8 mm) and cystic (CSTFF,>20 mm). Rabbit semen was collecte...

Abstract The incorporation of zinc in the diets breeding chickens to enhance reproductive performance has been highlighted. However, no consensus reached on effect dietary supplementation outcomes chickens. Therefore, current study assessed effects semen quality, fertility and hatchability percentage using meta-analysis. Furthermore, subgroup analysis was also used assess sources (organic versu...