The mRNA closed-loop, formed through interactions between the cap structure, poly(A) tail, eIF4E, eIF4G and PAB, features centrally in models of eukaryotic translation initiation, although direct support for its existence in vivo is not well established. Here, we investigated the closed-loop using a combination of mRNP isolation from rapidly cross-linked cells and high-throughput qPCR. Using th...

Abstrac t Digital subscriber lines (DSL) offer the possibility to deliver broadband services over the existing telephone network. Yet, before deploying DSL, the subscriber loops must be tested to see whether they can support high-speed data services, and at what rate. Single-ended automatic qualification is essential for achieving low-cost deployment of DSL, since it allows loops to be qualifie...

Plus-strand RNA viruses serve as templates for translation and then transcription by newly synthesized RdRp. A ribosome-binding tRNA-shaped structure (TSS) and upstream hairpin H4 in the 3' UTR of Turnip crinkle virus (TCV) play key roles in translation and transcription. Second-site mutations generated to compensate for altering the critical asymmetric internal loop of H4 included a three- to ...

HIV-1 Tat hijacks the human superelongation complex (SEC) to promote proviral transcription. Here we report the 5.9 Å structure of HIV-1 TAR in complex with HIV-1 Tat and human AFF4, CDK9, and CycT1. The TAR central loop contacts the CycT1 Tat-TAR recognition motif (TRM) and the second Tat Zn2+-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized in the TAR comp...

background: aging is a sensitive period of life and attention to the issues and needs is essential. to access to health care for the elderly and the prevention of chronic diseases, and reduce the incidence of infection, the first step is identifying the factors affecting the health of every community. one of the factors affecting it, is the social and religious support. thus, this study compare...

We consider the genus-one curves which arise in cuts of sunrise and elliptic double-box Feynman integrals. compute compare invariants these a number ways, including parametrization, lightcone Baikov (in full loop-by-loop variants). find that same geometry for arises all cases, lends support to idea there exists an invariant notion geometry, independent on way it is computed. further indicate ho...

Grid-forming converters (GFMCs) provide voltage support and other grid services, such as inertia droop, in more-electronics power systems. The GFMCs with small droop coefficients or connected to the through a impedance may have fast loop, which leads serious conflicts inner ac control resulting oscillations even instability. To address this problem, an extremely loop should be expected for GFMC...

The paper attempts to identify the impacts of five aspects of big data management competence in closed loop datafication system on service innovation performance and business performance in telecom industry in China. The research questions have been developed by integrating various theories (i.e. knowledge management theory, evolutionary perspective and resource-based theory) with the model of ...

In this article we develop the concept that the hippocampus and the midbrain dopaminergic neurons of the ventral tegmental area (VTA) form a functional loop. Activation of the loop begins when the hippocampus detects newly arrived information that is not already stored in its long-term memory. The resulting novelty signal is conveyed through the subiculum, accumbens, and ventral pallidum to the...

Generally, the robot manipulators are classified into serial type open loop chains and parallel type closed loop chains. Since decades, robot manipulators were designed and operated using the open loop serial chains. In general, Robots used in industries are all general purpose manipulators, consisting of multiple rigid links and are connected in series by means of joints such as revolute, pris...