Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of diff...

An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested...

Abstract Bioprospecting of anti-SARS-corona virus phytochemical agents is an important issue today as alternative to chemical drugs whose effectiveness has not been fully successful with no light effect. Quercetin, a component Toona sinensis young leaves extract, was reported have anti-viral activity against SARS-corona replication in vitro . Currently, the low efficiency secondary metabolite p...

Background: Ferula gummosa Boiss. is a medicinal and economical plant belonging to Apiaceae family. It is a native herb of Iran and is in serious danger of extinction. Objective: Gamma irradiation is an environmental stress and can consider as a new tool to change the content of secondary metabolites in medicinal plants. The effect of gamma irradiation on phenolic content and radical sc...

We report here a method for plant regeneration through somatic embryogenesis from explants collected from immature male inflorescence of adult oil palm cultivated in India. Callus induction was successful from tissues of immature male inflorescence collected from both dura and tenera varieties of oil palm. A modified Y3 (Eeuwens) media supplemented with several additives and activated charcoal ...

The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...

Perennial ryegrass (Lolium perenne L.) is a widely used cool-season grass species as turf and forage. In order to use biotechnological approaches to improve the species, factors affecting tissue culture responses of mature caryopses of perennial ryegrass were studied. Callus induction and regeneration from mature caryopses of thirteen recentlyreleased turf-type perennial ryegrass cultivars were...

In order to optimize callus induction in Pistacia atlantica Desf. subsp. kurdica Rech. f., the effect of different plant growth regulator (PGR) combinations were studied. Inflorescence axis explants were cultured on WPM medium supplemented with 1, 2 and 3 mg/L of TDZ or 1, 2 and 3 mg/L of BA, in combination with 1mg/l different auxins: 2, 4-D,  NAA  and IBA . Callus initiation was observed in a...

Abstract Pranajiwa (Euchresta horsfieldii (Lesch) Benn.) is an endangered medicinal plant known for its function as aphrodisiac, antidote, expectorant, and tonic. It also can be used to cure TBC. Research has found that pranajiwa difficult propagated, but the tissue culture propagation method never been applied this species. This research aimed figure out procedure induce callogenesis of with a...