By DAVID \V. \\ESTRING AND SUZANNE B. BnrrrlN T lIE HEMOLYTIC RESPONSE of erythrocytes to hypotonic stress is well known. Complex enzyme systems are required by the cell to maintam membrane integrity during exposure to hypotonic solutions. When certain of these enzymes are defective, erythrocyte osmotic fragility may be altered.3 On the other hand, the response of leukocytes to osmotic stress h...

The ubiquitin conjugating enzyme E2 (UBC E2) mediates selective ubiquitination, acting with E1 and E3 enzymes to designate specific proteins for subsequent degradation. In the present study, we characterized the function of the mung bean VrUBC1 gene (Vigna radiata UBC 1). RNA gel-blot analysis showed that VrUBC1 mRNA expression was induced by either dehydration, high salinity or by the exogenou...

BACKGROUND Escherichia coli suffer from osmotic stress during succinic acid (SA) production, which reduces the performance of this microbial factory. RESULTS Here, we report that a point mutation leading to a single amino acid change (D654Y) within the β-subunit of DNA-dependent RNA polymerase (RpoB) significantly improved the osmotolerance of E. coli. Importation of the D654Y mutation of Rpo...

Nitric oxide (NO) is a active molecule involved in mediation of various biotic and abiotic stressinduced physiological responses in plants. In the present study, using SNP (sodium nitroprusside) as NO donor and cPTIO [2-(4-carboxypheny-4,4,5,5-tetramethylimidazoline-1-1oxyl-3-oxide, potassium salt] as specific NO scavenger, we examined the ability of exogenous NO to alleviate oxidative damage, ...

The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. s...

Blue mussels (Mytilus edulis) are ecologically and economically important marine invertebrates whose populations are at risk from climate change-associated variation in their environment, such as decreased coastal salinity. Blue mussels are osmoconfomers and use components of the metabolome (free amino acids) to help maintain osmotic balance and cellular function during low salinity exposure. H...

We report here the isolation and functional analysis of AlTMP1 gene encoding a member of the PMP3 protein family. In Aeluropus littoralis, AlTMP1 is highly induced by abscisic acid (ABA), cold, salt, and osmotic stresses. Transgenic tobacco expressing AlTMP1 exhibited enhanced tolerance to salt, osmotic, H₂O₂, heat and freezing stresses at the seedling stage. Under greenhouse conditions, the tr...