We studied by the laboratory tests impact of most widespread pesticides (insecticide “Actor”, fungicide “Scooter”, herbicide “Titus-C”) in different concentrations (0,001–1000 mg/L) and water on main ecotoxicological indexes genetic vicaristic “western” “eastern” allospecies great ramshorn Planorbarius corneus s. lato. It was established, ranges toxic action these pollutants (mg/L) are followin...

Amyloid beta (Aβ) peptide aggregation is linked to the initiation of Alzheimer's disease; accordingly, aggregation-prone isoforms of Aβ, expressed in the brain, shorten the lifespan of Drosophila melanogaster. However, the lethal effects of Aβ are not apparent until after day 15. We used shibire(TS) flies that exhibit a temperature-sensitive paralysis phenotype as a reporter of proteostatic rob...

Previous analyses of Saccharomyces cerevisiae chromosome I have suggested that the majority (greater than 75%) of single-copy essential genes on this chromosome are difficult or impossible to identify using temperature-sensitive (Ts-) lethal mutations. To investigate whether this situation reflects intrinsic difficulties in generating temperature-sensitive proteins or constraints on mutagenesis...

In a study of the growth of chicken osteoblasts in cultures exposed to supranormal temperatures of 42 degrees C., 44 degrees C., 47 degrees C., 50 degrees C., and 52 degrees C., it has been observed that: 1. There is no lethal effect after prolonged exposures at 42 degrees C. and 44 degrees C. 2. Cultures are killed after an exposure of 105 minutes at 47 degrees C., of 6 minutes at 50 degrees C...

A pretreatment of the yeast, Candida oleophila, with 5 mM H(2)O(2) for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H(2)O(2)), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had...

BACKGROUND Neuraxial anesthesia reduces the shivering threshold approximately 0.6 degrees C. This effect might be mediated by an apparent (as opposed to actual) increase in lower body temperature. Accordingly, sufficient lower body warming should result in thermoregulatory inhibition comparable to that exerted by epidural anesthesia. We tested the hypothesis that increasing leg skin temperature...

Thirteen X-linked, cold-sensitive lethal, female-sterile mutants of Drosophila melanogaster located at eight separate loci were screened for their ability to assemble ribosomes at the restrictive temperature of 17 degrees. Females were labelled with 3H-uridine for either 2 or 20 hours at 17 degrees. A mitochondria-free extract was prepared and analyzed by means of sucrose gradient centrifugatio...

The acute and chronic toxicity of zinc to wild mottled sculpin (Cottus bairdi) was measured with 13-d and 30-d flow-through toxicity tests, respectively. Exposure water hardness was 48.6 mg/L as CaCO3 and 46.3 mg/L as CaCO3 in the acute and chronic tests, respectively; pH was slightly above neutral; and temperature near 12 degrees C. The median lethal concentration (LC50) after 96 h was 156 mic...

Climate change effects on population dynamics of natural populations are well documented at higher latitudes, where relatively rapid warming illuminates cause-effect relationships, but not in the tropics and especially the marine tropics, where warming has been slow. Here we forecast the indirect effect of ocean warming on a top predator, Nazca boobies in the equatorial Galápagos Islands, where...

The application of pulsed electric fields (PEFs) for the inactivation Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses range 25–40 kV/cm field intensities were applied at different frequencies (400–900 Hz) to investigate effect PEF conditions on microbial population proteins relevant this functional bevera...