The most important single factor affecting the efficiency of protein utilization is the profile of digestible essential amino acids entering the small intestine. Assuming a constant ideal amino acid profile of absorbed protein the requirements of all amino acids can be calculated when the requirement of one individual amino acid has been determined. In non-ruminants, the supply will be greatly ...

a thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. the solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. procedures in all the three stages were in the same dime...

Fungi produce α-aminoadipate, a precursor for penicillin and lysine via the α-aminoadipate pathway. Despite the biotechnological importance of this pathway, the essential isomerization of homocitrate via homoaconitate to homoisocitrate has hardly been studied. Therefore, we analysed the role of homoaconitases and aconitases in this isomerization. Although we confirmed an essential contribution ...

We conducted a series of investigations in order to elucidate role of nutritional status in regulating GLUT expression and energy metabolism in porcine muscle. Firstly, the role of mild undernutrition in regulating muscle GLUT gene expression and function was studied in growing pigs (3 wk of age) on a high (H) or low (L) food intake (H = 2L) at 35°C or 26°C. Low food intake selectively upregula...

HOZOVÁ B., JANČOVIČOVÁ J., DODOK L., BUCHTOVÁ V., STARUCH L. (2002): Use of transglutaminase for improvement of quality of pastry produced by frozen-dough technology. Czech J. Food Sci., 20: 215–222. The improving effect on the quality of final products has been studied of the enzyme transglutaminase added in two concentrations, i.e. 4.5 mg or 7.5 mg/300 g of flour destined for the preparation ...

The correlation between protein synthesis and the nuclear division cycle in Neurospora crassa hyphae was studied by inhibiting protein accumulation by two different experimental procedures: (1) starvation for lysine in a lysine-requiring mutant (lys-1); and (2) addition of cycloheximide. Lysine starvation in a lys-1 strain of N. crassa quickly blocked the nuclear division cycle and nuclei accum...

I. The nutritive value of solubilized wool protein prepared by dissolving finely ground wool in sodium sulphide-sodium sulphite solution followed by precipitation with 40 % acetic acid has been tested by incorporating it into protein-free diets containing the amounts of vitamins and minerals required by weanling rats. 2. At the level of 10 % (w/w) of the diet, solubilized wool protein failed to...

1. In hay-fed, cannulated sheep the apparent degradation in and outflow from the rumen were determined for graded doses of mixtures of the amino acids lysine, threonine and methionine, administered intraruminally and using polyethylene glycol (PEG) as a liquid marker. The doses ranged between 2.5 and 15 g for each amino acid in the mixtures. 2. Relative rate of apparent degradation in the first...

Serum urea concentrations were measured in Large White pigs from lines divergently selected for components of efficient lean growth rate and performance tested over three 14-d test periods starting at 30, 50, and 75 kg. Two methods of performance testing were used. Phase-fed pigs were fed to appetite isoenergetic diets differing in total lysine:energy ratio (0.58, 0.69, 0.81, 0.91, 1.01, 1.12, ...