Acanthamoeba releases lysosomal hydrolases continuously into the culture medium. This release is specific for lysosomal hydrolases, but not other cellular proteins, and is energy dependent. The secreted hydrolases can be separated into two groups on the basis of their secretion kinetics: one is secreted at approximately 15% of the cellular activity per hour and the other at approximately 5%. In...

The rabbit corneal endothelium phagocytized polystyrene spheres 0.5 Mm in diameter. After phagocytizing spheres, the endothelium degenerated, and lost from the Descemet's membrane. Lysosomal enzyme activities of the endotheliumDescemet's membrane complex, such as acid phosphatase, /?-glucuronidase and N-acetyl-/?-D-glucosaminidase, were assayed and the total activities per microgram DNA were al...

Oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. Cadmium (Cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. Because of its long biological half-life (10–30 years), Cdaccumulates in the biological systems. The present study was designed to assess the concen...

four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of turkmen stallion's sperm. collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 u/ml (cat100), 150u/ml (cat150) and 200u/ml (cat200)]. exten...

the purpose of this study was to determine the effects of different concentrations of vitamin e (vit. e) in the quality of preserved stallion semen at 5ᵒc for a period of 48 h. semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of vit. e...

antioxidant effect of olive leaf extract (ole) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 ross 308 roosters at their 30 weeks of age. semen samples were collected by abdominal massage in 5 times. in each session after the initial sperm assessment, collected samples were pooled and diluted with sexton extender.samples were sp...

Lysosomes were purified approximately 40-fold from rat kidney cortex by differential and Percoll density gradient centrifugation. In a sucrose medium, the lysosomes quenched the fluorescence of the potential sensitive dye diS-C3-(5) (3,3'-dipropylthiocarbo-cyanine iodide) in a time-dependent manner, indicating that the dye accumulates within the lysosomal interior. After treatment of the lysoso...

The tumor suppressor wild-type p53 can induce apoptosis. M1-t-p53 myeloid leukemic cells have a temperature-sensitive p53 protein that changes its conformation to wild-type p53 after transfer from 37 degrees C to 32 degrees C. We have now found that these cells showed an early lysosomal rupture after transfer to 32 degrees C. Mitochondrial damage, including decreased membrane potential and rele...

BACKGROUND Clinical diagnosis of parkinsonian syndromes like Parkinson's disease (PD), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP) is hampered by overlapping symptomatology and lack of diagnostic biomarkers, and definitive diagnosis is only possible post-mortem. OBJECTIVE Since impaired protein degradation plays an important role in many neurodegenerative disorder...

γ-l-glutamyl-S-[2-[[[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl]oxy]carbonyl]-3-[[2-(1H-indol-3-yl)ethyl]amino]-3-oxopropyl]-l-cysteinylglycine sodium salt (ESeroS-GS) is a water-soluble derivative of α-tocopherol (vitamin E). We reported previously that ESeroS-GS can act as an anti-inflammatory agent and can induce cell death in breast cancer cells. Howev...