AIM To use parallel array pyrosequencing to deconvolute mixtures of mitochondrial DNA (mtDNA) sequence and provide high resolution analysis of mtDNA heteroplasmy. METHODS The hypervariable segment 1 (HV1) of the mtDNA control region was analyzed from 30 individuals using the 454 GS Junior instrument. Mock mixtures were used to evaluate the system's ability to deconvolute mixtures and to relia...

BACKGROUND AND AIMS Current understanding of stomatal development in Arabidopsis thaliana is based on mutations producing aberrant, often lethal phenotypes. The aim was to discover if naturally occurring viable phenotypes would be useful for studying stomatal development in a species that enables further molecular analysis. METHODS Natural variation in stomatal abundance of A. thaliana was ex...

The oldest extant human maternal lineages include mitochondrial haplogroups L0d and L0k found in the southern African click-speaking forager peoples broadly classified as Khoesan. Profiling these early mitochondrial lineages allows for better understanding of modern human evolution. In this study, we profile 77 new early-diverged complete mitochondrial genomes and sub-classify another 105 L0d/L...

The Zika virus (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described sexually and vertically transmitted. latter shows severe consequences for the embryo/fetus, such congenital microcephaly deficiency of neural system, currently known Congenital Syndrome (CZS). To better understand dynamics in trophoblastic cells present first trimester pr...

young children with history of maternal stress during pregnancy suffer from several neurological and psychological disorders in the childhood. the purpose of this study was to investigate the effect of maternal stress during pregnancy on the neural apoptosis in the newborns. pregnant wistar rats were divided into control and stress groups. stress was applied on the pregnant females from day 15 ...

conclusions smm analysis provides valuable information on obstetric care. this study highlighted the need to improve antenatal care, which would help early identification of high-risk pregnancies. every smm could progress to maternal mortality if is not suspected, diagnosed and managed on its golden time. background investigating severe maternal morbidity/near miss is a newly recognized tool wh...

introduction maternal and fetal characteristics can be effect on optimism which vary among different cultures and countries. so the aim of this study was to determine prenatal optimism and its relationship with fetal and maternal characteristics in primiparous women. materials and methods this research study was a descriptive cross - sectional. the statistical populations consisted of 240 pregn...