The prognosis of patients with metastasised follicular thyroid carcinoma (FTC) is limited, necessitating the search for new treatment options. Beneficial effects of retinoids have been suggested in thyroid cancer and the present study was performed to investigate the effects of retinoic acid (RA) on important determinants of metastatic behaviour in FTC: the disengagement of tumour cells from th...

Several metabolic processes, such as DNA organization and replication, transcription, and RNA processing are closely associated with the nuclear matrix. Nuclear matrix attachment regions are nucleotide sequences holding DNA tightly complexed with the nuclear scaffold and are resistant to extractions with detergents and high salt solutions. The role of matrix attachment regions in DNA replicatio...

The yeast ARS-1 element contains a scaffold attachment region (SAR) that we have previously shown can bind to plant nuclear scaffolds in vitro. To test effects on expression, constructs in which a chimeric beta-glucuronidase (GUS) gene was flanked by this element were delivered into tobacco suspension cells by microprojectile bombardment. In stably transformed cell lines, GUS activity averaged ...

   To fabricate a defect free and high performance mixed matrix membrane (MMM), one approach is the functionalization of inorganic nanofillers (as dispersed phase) in the organic polymer matrix (as continuous phase) to modify the interactions between two phases.  For this purpose,, raw multi-walled carbon nanotubes (rMWCNTs) were purified by acid mixture (HNO3/H2SO<sub...

One of the major hurdles of isolating stable, inducible or constitutive high-level producer cell lines is the time-consuming selection procedure. Given the variation in the expression levels of the same construct in individual clones, hundreds of clones must be isolated and tested to identify one or more with the desired characteristics. Various boundary elements (BEs), matrix attachment region...

Using competitive binding experiments, it was found that native type XI collagen binds heparin, heparan sulfate, and dermatan sulfate. However, interactions were not evident with hyaluronic acid, keratan sulfate, or chondroitin sulfate chains over the concentration range studied. Chondrocyte-matrix interactions were investigated using cell attachment to solid phase type XI collagen. Pretreatmen...

Interactions between cell-surface integrins and extracellular matrix proteins underlie a versatile recognition system providing cells with anchorage, traction for migration or matrix remodeling, as well as signals for polarity, differentiation and growth. Short peptide sequences of fibronectin (FN), most notably RGD found on a loop in the 10th type III domain, are effective in promoting cell ad...