Batch and fed-batch cultivation of Bacillus thurillgiellsis var. kurstaki was carried out to achieve high cell and spore density with maximum insecticidal activity. Cell cultivation was done in fully equipped fermentors under controlled conditions at different feed rates. Increased cell mass, high spore counts and improved insecticidal activity were achieved with fed-batch cultivation system. W...

A five-step method is proposed for determining the design space of mesenchymal stem cell (MSC) cultivation processes incorporating system dynamics and variabilities. The uses mechanistic models to capture MSC while performing stochastic simulation consider influences variabilities at raw material, cell, process specification, operation levels. Simulated time-dependent changes in critical qualit...

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...

Under increased oxygen tensions, Thermus aquaticus exhibited a lag period in growth of 80 min, during which the specific activities of catalase, peroxidase, and superoxide dismutase increased. Methyl viologen increased the lag period, decreased the maximum population density, increased cell lysis, and induced catalase and superoxide dismutase. Methyl viologen, in conjunction with chloramphenico...

introduction: cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components. methods: clonogenic assay was used for assessment of cell ...

BACKGROUND Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. METHODS Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static...