The aim of this study was to investigate the applicability of an SPR biosensor in combination with a -lactam receptor protein for generic detection of -lactam antibiotics in milk. β-Lactam antibiotics constitute the group of antimicrobials most commonly used for treatment of bacterial infections in dairy cows. Consequently, they are also the most common type of drug residue found in milk and, a...

MRL/MpJ-Fas(lpr) (MRL-Fas(lpr)) mice develop a spontaneous T cell and macrophage-dependent autoimmune disease that shares features with human lupus. Interactions via the programmed death 1/programmed death ligand 1 (PD-1/PD-L1) pathway down-regulate immune responses and provide a negative regulatory checkpoint in mediating tolerance and autoimmune disease. Therefore, we tested the hypothesis th...

Complement receptor 1-related gene/protein y (Crry) in rodents is a potent membrane complement regulator that inhibits complement C3 activation by both classical and alternative pathways. Complement inhibition with Crry as the recombinant protein Crry-Ig has been demonstrated to prevent MRL/MpJ-Tnfrsf6(lpr) (MRL/lpr) mice from developing proteinuria and renal failure. Crry-Ig-treated mice also ...

Abstract Wild-type Murphy Roth Large (MRL) mice have long been investigated for their superior healing ability when subjected to various wound and disease models. Despite this long history, the mechanisms causing their extraordinary healing ability remain undefined. As we have recently demonstrated that MRL mice with muscular dystrophy are resistant to the associated fibrosis and the Heber-Katz...

Within the particular context of food safety, the concentrations of pesticides that have been detected should absolutely be lower than the authorized maximum concentration level (MRL). In that respect, the availability of complete data is of the utmost importance for deciding whether a product is or is not compliant with the regulations in force. However, these concentrations only get their ful...

PURPOSE MRL/MpJ-fas+/fas+ (MRL/+) and MRL/MpJ-fas(lpr)/fas(lpr) (MRL/lpr) mice undergo spontaneous development of inflammation of the lacrimal and salivary glands, similar to that in the human disorder Sjögren's syndrome. Previous work has shown that these lesions appear to be largely T helper (Th)-2-driven, as evidenced by the substantially greater expression of IL-4 than interferon-gamma. The...

Congenic mice of the MRL/Mp strain spontaneously develop an autoimmune connective tissue disease that shares immunologic and histopathologic features with the human disorders systemic lupus erythematosus, rheumatoid arthritis, and systemic vasculitis. The autoimmune disorder in these mice is markedly accelerated by the recessive gene lpr. Older MRL/Mp-lpr/lpr mice develop significant inflammato...

Glyphosate (N-phosphonomethyl glycine) is one of the most widely used pesticide around the world. It is very well known that glyphosate compound rapidly degrades into aminomethylphosphonic acid (AMPA) metabolite (Fig. 1). Glyphosate is a herbicide with a broad spectrum of activity, very effective even on plant roots with little harmful effects on mammals. Its high efficacy, low toxicity and aff...

Infection with gram-negative and gram-positive bacteria remains a leading cause of death in patients with systemic lupus erythematosis (SLE), even in the absence of immunosuppressive therapy. To elucidate the mechanisms that underly the increased risk of infection observed in patients with systemic autoimmunity, we have investigated host defense against bacterial infection in a murine model of ...

Antibiotics are considered an important primary therapy for bacterial diseases in aquaculture. This study evaluated the influence of oral administration oxytetracycline (OTC) on feed intake, growth, mortality, residue accumulation and clearance, histopathological changes vital organs six groups Nile tilapia Oreochromis niloticus when fed at 0–10 times therapeutic dose (1×: 80 mg/kg biomass/day)...