AIM/PURPOSE OF THE STUDY To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. MATERIALS AND METHODS Human limbal explants were cultured on intact human amniotic membranes for two weeks. The sheets were stored in a hermetically sealed container at 23°C in either a serum-fre...

Since Acholeplasma laidlawii can be restricted to incorporating fatty acids from the growth medium into its membrane lipids, it is possible to study the effects of the length of the acyl chains on the properties of the membrane of the organism. A. laidlawii strain A-EF22 was grown with mixtures of one perdeuterated saturated fatty acid and one monounsaturated fatty acid. The average length (<Cn...

The effective thickness of the unstirred fluid layer (USL) adjacent to an epithelial barrier can be estimated from the time course for the accumulation or depletion of a solute at the membrane surface. In 1985 we reported an unstirred layer thickness of approximately 70 microns for Necturus gallbladder epithelium. In our earlier studies the delay caused by noninstantaneous bulk solution mixing ...

Transmission electron microscopy of ultrathin sections was used to measure mouse retinal capillary basement membrane thickness (BMT). BMT increases predictably with age from 50 +/- 9 nm at 6 weeks to 154 +/- 27 nm at 20 months, but is not affected by strain or sex. There is an effect of retinal site, however, with BMT increasing with the radial distance from the optic nerve, 41 +/- 4 nm (center...

PURPOSE To assess a potential role of Bruch´s membrane (BM) in the biomechanics of the eye, we measured its thickness and the density of retinal pigment epithelium (RPE) cells in various ocular regions in eyes of varying axial length. METHODS Human globes, enucleated because of an ocular tumor or end-stage glaucoma were prepared for histological examination. Using light microscopy, the histol...

PURPOSE To evaluate the effect of repeated intravitreal ranibizumab injections for neovascular age related macular degeneration (nAMD) on the retinal nerve fiber layer (RNFL) thickness using optical coherence tomography. DESIGN A prospective observational cohort study of patients with nAMD. METHODS Thirty eyes of 30 patients with nAMD were selected. All patients received three ranibizumab i...