Computational methods in protein mass spectrometry, DNA microarray technology and protein folding. Dedication To my mother Ludmila and my wife Jennifer.

M icroarrays are used to survey the expression of thousands of genes in a single experiment. Applied creatively, they can be used to test as well as generate new hypotheses. As the technology becomes more accessible, microarray analysis is finding applications in diverse areas of biology. Microarrays are simply a method for visualizing which genes are likely to be used in a particular tissue at...

This paper describes the technology and performance characteristics of the Oligo GEArray® DNA microarray system. Oligo GEArrays® are an effective alternative to genome-wide microarrays, in that each array is designed to profile expression of a focused gene panel from a particular biological pathway or disease state. The data presented in this paper substantiates the sensitivity, reproducibility...

Many applications of modern science involve a large number of parameters. In many cases, the number of parameters, p, exceeds the number of observations, N . Classical multivariate statistics are based on the assumption that the number of parameters is fixed and the number of observations is large. Many of the classical techniques perform poorly, or are degenerate, in high-dimensional situation...

This review discusses recent advances in our understanding of the human genome and the application of derived technologies in the medical area. It focuses on the use of DNA microarray for diagnosis, prognosis, and therapeutic purposes in oncology, and the resultant impact it might have on routine clinical practice.