Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted bacteria. These arrays were used in a series of bacterial immunoassays to detect E. coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (μCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that ta...

One difficulty in studying epithelial tumors has been the inability to isolate pure samples for DNA and RNA analysis. Prostate cancer, with its infiltrative nature, is particularly challenging. The Combination of several new technologies should help overcome these hurdles. Laser capture microdissection uses a laser beam to achieve transfer of pure cell populations for isolation of DNA, RNA, and...

It is of great importance to efficiently immobilize probes onto a substrate with good spot quality for fabrication of protein microarrays. Printing buffers play an essential role in the fabrication process for the microarrays. In this work, antigen (Ag)/antibody (Ab) microarrays were fabricated on 3-aminopropyltriethoxysilane (APTES) modified glass slides through glutaraldehyde (GA), a bis-alde...

High-throughput microarray technologies have the potential to impact significantly on the practice of histopathology over the coming years. Global gene expression profiling allows for a systematic search of all human genes for novel diagnostic and prognostic markers and for potential therapeutic targets. Likewise, gene copy number changes can be determined on a gene-by-gene basis using microarr...