Most neurons fire in bursts, imposing episodic energy demands, but how these demands are coordinated with oxidative phosphorylation is still unknown. Here, using fluorescence imaging techniques on presynaptic termini of Drosophila motor neurons (MNs), we show that mitochondrial matrix pH (pHm), inner membrane potential (Δψm), and NAD(P)H levels ([NAD(P)H]m) increase within seconds of nerve stim...

Electroencephalography (EEG) is an effective, efficient, and noninvasive method of assessing and recording brain activity. Given the excellent temporal resolution, EEG can be used to examine the neural response related to specific behaviors, states, or external stimuli. An example of this utility is the assessment of the mirror neuron system (MNS) in humans through the examination of the EEG mu...

Hypoglossal motoneurons (XII MNs) control muscles of the mammalian tongue and are rhythmically active during breathing. Acetylcholine (ACh) modulates XII MN activity by promoting the release of glutamate from neurons that express nicotinic ACh receptors (nAChRs). Chronic nicotine exposure alters nAChRs on neurons throughout the brain, including brain stem respiratory neurons. Here we test the h...

Stem cell-derived motor neurons (MNs) are increasingly utilized for modeling disease in vitro and for developing cellular replacement strategies for spinal cord injury and diseases such as spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). Human embryonic stem cell (hESC) differentiation into MNs, which involves retinoic acid (RA) and activation of the sonic hedgehog (SHH) p...

Facial expressions and hand gestures are utilized in nonverbal communication to convey socially relevant information. One key process that mediates nonverbal communication is simulation. The mirror neuron system (MNS), which maps observed actions onto the motor representations used when producing those actions, likely plays a role in simulation. Previous neuroimaging experiments have identified...

An experimental investigation has been conducted with respect to the influence of permanent magnet stirring (PMS) under different rotation speeds (0, 50, 150 rpm) a center magnetic flux density 1450Gs on evolution MnS precipitation, microstructure, and mechanical properties high-sulfur micro-alloyed 49MnVS3 steel. The thermodynamic calculation results indicated that began precipitate in 1419 °C...

This study introduces novel aspects of inward rectification in neonatal rat spinal motoneurons (MNs) and its modulation by serotonin (5-HT). Whole cell tight-seal recordings were made from MNs in an isolated lumbar spinal cord preparation from rats 1-2 days of age. In voltage clamp, hyperpolarizing step commands were generated from holding potentials of -50 to -40 mV. Discordant with previous r...

Background Atopic dermatitis (AD) is a chronic inflammatory skin disease whose pathogenesis, cause, and treatment have been extensively studied. The association of AD with Th2 cytokines well known; therefore, the analysis this crucial for diagnosis AD. This study aimed to present new method measuring protein biomarkers in patients AD, before after treatment, using minimally invasive microneedle...

Matching Needs and Services (MNS) is a practice tool intended to help people who work with vulnerable children use rigorously assembled information on needs as a guide to design, implement, and evaluate more-effective services. To do this, MNS focuses on needs but links them to outcomes and thresholds before dealing with the services to achieve those outcomes.