Two years ago, a modification (1) of the Bell-Doisy phosphate method was published and at that time technique ‘was given for its application to inorganic phosphorus in blood and urine. Since then its use has been extended to a variety of analytical procedures, some of which are given below. In principle the method consists of the formation of phosphomolybdic acid and its subsequent reduction by...

The molybdate-stabilized calf uterine estradiol receptor has been purified to near-homogeneity by a three-step procedure. Initial purification by heparin-Sepharose chromatography provides a concentrated receptor extract in 40% yield with a 5-10-fold increase in purity. The inclusion of molybdate in phosphate-buffered cytosol enhances 9-10 S receptor stability in high salt and allows elution of ...

Rhizobia-legume symbiosis is the most well researched biological nitrogen fixation system. Coating legume seeds with rhizobia is now a recognized practical measure for improving the production of legume corp. However, the efficacy of some commercial rhizobia inoculants cannot be guaranteed in China due to the low rate of live rhizobia in these products. A greenhouse experiment was conducted to ...

Frequent observations have been made of the increased rate of hydrolysis of relatively labile phosphate compounds in acid molybdate solution (e.g. phosphocreatine (I), acetyl phosphate (2), and 1,3-diphosphoglyceric acid (3)). A study by Weil-Malherbc and Green (4) indicated that ATP, pyrophosphate, phosphoenolpyruvate, and fructosr B-phosphate were hydrolyzed appreciably in the pH range 3 to 5...

The molybdate-dependent transcriptional regulator (ModE) from Escherichia coli functions as a sensor of molybdate concentration and a regulator for transcription of operons involved in the uptake and utilization of the essential element, molybdenum. We have determined the structure of ModE using multi-wavelength anomalous dispersion. Selenomethionyl and native ModE models are refined to 1. 75 a...

An indirect method for the determination of silicon in blood samples has been developed. The proposed method overcame interference from a large amount of salts and phosphate in blood samples, and enabled us to determine the silicon contents in serum and whole blood by the same operation. After blood samples were digested by microwave heating, silicon, present as silicate in the sample solution,...

the quenching of quinine fluorescence intensity in the presence of some anions in aqueous solution at ambient temperature has been investigated. the quenching is found to be collisional or dynamical in nature. this study reveals the order of two groups of quencher: nai > nabr > nacl > naf and k2cr2o7 > kmno4 >na2so4 > naclo3. increasing anion size in the both groups leads to an  increase in the...

Metal molybdate-doped aluminium phosphate heterostructures have been prepared by a solid solution method and found to be photoluminescence, semiconducting properties effective catalysts for the photoreduction of o -nitroaniline p -nitroaniline.