Monoclonal antibody directed against RNA polymerases IIO and IIA completely inhibits specific transcription from the major late promoter of adenovirus-2. At comparable antibody concentrations, only a slight inhibition of polymerase III transcription of VA RNA I is observed. Inhibition of polymerase II transcription is specific for monoclonal IgM directed against RNA polymerase II. High concentr...

Levilimab is a monoclonal antibody targeting interleukin-6 receptors and having potent anti-inflammatory activity. Efficacy safety of levilimab for treatment rheumatoid arthritis were established in multicenter, double blind, placebo-controlled clinical trials AURORA SOLAR. The authors present two patients with who treated successfully discuss various issues its use practice.

Monoclonal mouse antibody to streptococcal group A carbohydrate was evaluated in diagnostic microbiology laboratory. A total of 262 isolates of beta-hemolytic streptococci were classified with commercial reagents and the monoclonal antibody to group A carbohydrate by using immunofluorescence and Lancefield precipitin tests. Both nonoclonal and commercial antibodies were identifically reactive b...

Four monoclonal antibodies from euthymic mice and two monoclonal antibodies from athymic mice were directed against antigens of Rickettsia conorii, as shown by both indirect immunofluorescence and an enzyme immunoassay. There was extensive cross-reactivity with other spotted fever group rickettsiae. Euthymic monoclonal antibodies 3-2 and 9-2 (immunoglobulin G2a [IgG2a]) and 27-10 (IgG1) distinc...

Detection of administered human monoclonal antibodies in the tissues and circulation of patients requires special reagents to overcome interference by normal endogenous immunoglobulin. A practical approach is the development of antiidiotypic antibodies to the human monoclonal antibody and their application in immunoassays specific for the human monoclonal antibody. Accordingly, antiidiotypic an...

We describe the production and characterization of a monoclonal antibody specific for platelets. This antibody reacts strongly with human and primate platelets, but does not recognise human monocytes, polymorphonuclear leucocytes, lymphocytes, erythrocytes, leukaemic nor fibroblast cell lines, nor rodent platelets. Immunoprecipitation studies using radiolabelled platelet membrane proteins showe...

An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either met...

A monoclonal antibody directed against a 65-kDa brain protein demonstrates an epitope found in keratin from human epidermis. By indirect immunofluorescence, the antibody decorates intracytoplasmic filaments in a subclass of astrocytes and Purkinje cells of adult hamster brain. Double-label immunofluorescence study using antibody to glial fibrillary acidic protein and this antibody reveals the 6...