Osteoclasts are derived from macrophage-lineage precursors. ED1 is an antibody that can recognize this lineage of cells. Matrix metalloproteinase 9 (MMP9) is essential for the migration of osteoclasts and their precursors during osteoclastogenesis. The aim of this research was to investigate differentiation and recruitment of osteoclasts during the early phase of experimental tooth movement in ...

IT MAY BE CONCLUDED THAT, UNDER THE CONDITIONS OF THE EXPERIMENTS: 1. Lymphocytes and large mononuclear cells can live and increase greatly in numbers in blood serum, while fibroblasts are not capable of doing so. 2. While living in serum, lymphocytes and large mononuclear cells manufacture and secrete substances which may be used as food material by the fibroblasts. 3. It is probable that lymp...

Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, C...

Human peripheral blood mononuclear cells (lymphocyte-monocyte) in culture release a solube factor which can stimulate, up to 200-fold, production of prostaglandin E2 by isolated, adherent, rheumatoid synovial cells. Production of the factor by the mononuclear cells is enhanced by phytohemagglutinin. This factor is similar in apparent mol vt (10,000-20,000) to that which also stimulates collagen...

Many patients with COVID-19 experience a range of debilitating symptoms months after being infected, syndrome termed long-haul COVID. A 68-year-old male presented lung opacity, fatigue, physical and cognitive weaknesses, loss smell lymphocytopenia. After rounds therapeutic plasma exchange (TPE), the patient returned to normal activities work. Mechanistically in patient’s peripheral blood...

Bronchial asthma is associated with eosinophilic inflammation and expression of T-cell-derived cytokines, which influence eosinophilic function. FK506, a newly established immunosuppressive agent, may have potential as a therapeutic instrument for asthma because of its suppressive effect on T-cell activation. To assess this, we compared the inhibitory effects of FK506 and cyclosporin A on produ...

AIM To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to dete...

Introduction: According to the cumulative evidence, genes encoding GABA receptors inhibit neurotransmitters in CNS and are intricately involved in the pathogenesis of mood disorders. Based on this hypothesis, these genes may be expressed in bipolar patients. As a result, we evaluated the gene expressions of GABA-β3 and HT1D receptors to assess their associations with bipolar mood disorder....

Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% +/- 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30-ml volume. Analysis of sequential 5-ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenit...

Dipeptidyl peptidase IV (CD 26 leukocyte differentiation antigen) is an enzymic surface marker of a human T lymphocyte subpopulation which has been shown to be associated with their capacity to produce large amounts of interleukin 2 and proliferate strongly in response to mitogenic stimulation. The peptidase activity on the surface of purified human peripheral mononuclear cells was determined s...