REPEATED intravenous injections of sodium iodoacetate produce a characteristic visual cell degeneration in the monkey, cat, and rabbit (Schubert and Bornschein, 1951; Noell, 1952), the resulting histological picture being very similar to that presented in human retinitis pigmentosa (Noell, 1953). Recently, it has been shown that a similar condition can be produced in the rat by careful injectio...

Saccharopine dehydrogenase (e-N-(L-glutaryl-2)-L1ysine:NAD oxidoreductase (L-lysine-forming) EC 1.5.1.7) is inhibited by a variety of sulfhydryl reagents. Althoughp-chloromercuribenzoate and 5,5’-dithiobis(2nitrobenzoate) react with three sulfhydryl groups present in the enzyme, reaction with one reactive sulfhydryl group leads to complete loss of catalytic activity. Amino acid analysis of the ...

We examined the methodological approach to the assessment of monosodium glutamate intake. The high carbohydrate and low fat consumption characteristic of this study population would be conducive to the development of metabolic syndrome. However, anomalies in the assessment of dietary information limits conclusion to a causal link of monosodium glutamate to metabolic syndrome and overweight beca...

The reaction of fumarase with several alkylating agents has been examined. The structural similarity between these compounds and the natural substrates for the enzyme, fumarate and malate, suggested that reaction might occur with structures in the active sites. Of all the compounds tested, only iodoacetate and 4-bromocrotonate inactivated fumarase by modification of structures other than the th...

A novel microperifusion system with capabilities for continuous, real-time, potentiometric monitoring of extracellular hydrogen ion concentration has been used to define the response of HeLa cells to abrupt changes in extracellular energy sources or introduction of an inhibitor of glycolysis. Glycolytic inhibition, induced by removal of glucose or introduction of iodoacetate, each led to a rapi...

The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of os...

The style component of the self-incompatibility (S) locus of the wild tomato Lycopersicon peruvianum (L.) Mill. is an allelic series of glycoproteins with ribonuclease activity (S-RNases). Treatment of the S3-RNase from L. peruvianum with iodoacetate at pH 6.1 led to a loss of RNase activity. In the presence of a competitive inhibitor, guanosine 3'-monophosphate (3'-GMP), the rate of RNase inac...

Prolonged constant light exposure causes disruptions in circadian rhythms, resulting in splitting of circadian activity rhythms in hamsters and arrhythmicity in rats. Hamsters with lesions of the thalamic intergeniculate leaflet do not exhibit constant light-induced disruptions in rhythmicity. We have shown that circadian rhythms of rats with monosodium glutamate-induced neurotoxic damage to vi...

This study was designed to evaluate the neuroprotective activity of ethanol extract of Pongamia pinnata stem bark in monosodium glutamate-induced neurotoxicity in rats. Neurotoxicity was induced by intraperitoneal injection of monosodium glutamate 2 g per kg body weight daily for 7 days. Ethanol extract of Pongamia pinnata stem bark (200 and 400 mg/kg) was administered orally after 1 h of monos...