The Rockefeller University Press $30.00 J. Cell Biol. Vol. 196 No. 4 483–496 www.jcb.org/cgi/doi/10.1083/jcb.201110114 JCB 483 K. Moreau and B. Ravikumar contributed equally to this paper. Correspondence to David C. Rubinsztein: [email protected] Abbreviations used in this paper: 5ase, 5-phosphatase; Arf, ADP ribosylation factor; ARNO, Arf nucleotide binding site opener; Baf A1, bafilomy...

results in vitro analysis showed that the greater the concentration of the extract, the greater the cytotoxicity. conclusions the crude extract of p. australis might be cytotoxic at high concentrations as evaluated against the cell line. materials and methods the mtt cytotoxicity assay was performed on a 3t3 mouse fibroblast cell line to measure the in vitro cytotoxic activity of the crude form...

The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate iPS cells with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. GIBCO media and reagents have been at the forefront of pluripotent stem cell research for years. Knockout DMEM supplemented with Kn...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 204 No. 7 1191–1207 www.jcb.org/cgi/doi/10.1083/jcb.201309107 JCB 1191 Correspondence to Keiko Kawauchi: [email protected]; or kawauchi30@ gmail.com S. Yamauchi’s present address is Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan. K. Kawauchi’s present address is Frontiers of Innovative Research in Science and Tec...

In vitro studies evaluating the cytotoxic potential of substances released from dental adhesives are lacking. The purpose this study was to compare cytotoxicity extracts Scotchbond Universal and Optibond Solo Plus, an adhesive in experimental phase: T1. 3T3 mouse fibroblast cells MG-63 osteoblast-like human osteosarcoma were exposed for 24 h serial extract dilutions. Cytotoxicity determined usi...

The Arp2/3 complex nucleates the formation of the dendritic actin network at the leading edge of motile cells, but it is still unclear if the Arp2/3 complex plays a critical role in lamellipodia protrusion and cell motility. Here, we differentiated motile fibroblast cells from isogenic mouse embryonic stem cells with or without disruption of the ARPC3 gene, which encodes the p21 subunit of the ...

Rationale: Fibroblast growth factor homologous factors (FHFs), a subfamily of fibroblast growth factors (FGFs) that are incapable of functioning as growth factors, are intracellular modulators of Na channels and have been linked to neurodegenerative diseases. Although certain FHFs have been found in embryonic heart, they have not been reported in adult heart, and they have not been shown to reg...

Background: The fibroblast growth factors (FGF-4) are a group of heparin-binding single chain polypeptides that play a pivotal role in development, cell growth, tissue repairing and transformation. The aim of this study is to improve development and maturation outcome of vitrified mouse pre-antral follicle by adding of fibroblast growth factor (FGF) into medium culture. Materials and Methods: P...