The effect of total body irradiation (5 Gy) on functional mouse erythroid lineage has been studied. The transferrin binding capacity by bone marrow cells and the activity of glycolytic regulatory enzymes and intracellular levels of 2,3 bisphosphoglycerate in peripheral blood erythrocytes have been determined. Results obtained along one year post-irradiation period suggest a complete recovery in...

Peripheral blood eosinophils from patients with eosinophilia and from healthy subjects were studied for surface immunoglobulins, receptors for the Fc region of IgG, complement receptors, and spontaneous rosette formation with sheep and mouse erythrocytes. Eosinophils were found to have receptors for complement and for aggregated IgG, and to have the same two types of complement receptors as do ...

mammary cancer than those from nontumorous animals. The question arose : Are these findings characteristic only for red blood cells from CSH mice with mammary cancer, or are these findings also valid for other mouse strains and other tu mors, including transplants of tumors originally induced with methylcholanthrene? Experiments were performed to investigate the hemolytic behavior of erythrocyt...

The new antimalarial drug mefloquine bound with high affinity (Kd approximately 3 X 10-7 M) to membrane lipids of normal mouse erythrocytes and of erythrocytes infected either with chloroquine-susceptible or chloroquine-resistant Plasmodium berghei. Approximately 80 nmol of mefloquine was bound per mg of total lipid. Mefloquine also bound to purified phospholipids with high affinity (Kd approxi...

Beta-caryophyllene (BC) is a chemical found in the essential oil of numerous plants which have shown various biomedical properties. In the present investigation we examined for the first time its genotoxic capacity in vivo by evaluating its potential to induce micronuclei in mouse. In a first assay we administered three doses of the compound once, and in a second assay, we administered three do...

The effects of medium conditioned by erythrocytes on in vitro differentiation of mouse prenatal erythroblasts were examined. Haem synthesis was inversely proportional to the concentration of erythrocyte-conditioned medium (ECM) in either presence or absence of erythropoietin (EP), but concentrations of ECM which limited haem synthesis to 50% of normal had no effect on changes in erythroblast nu...

The pathogenesis of malaria, an insect-borne disease that takes millions of lives every year, is still not fully understood. Complement receptor 1 (CR1) has been described as a receptor for Plasmodium falciparum, which causes cerebral malaria in humans. We investigated the role of CR1 in an experimental model of cerebral malaria. Transgenic mice expressing human CR1 (hCR1(+)) on erythrocytes we...

Phagocytic leukocytes have on their plasma membranes receptors for the Fc portion of immunoglobulin G (IgG) (1-9) and for a cleavage product of the third component of complement (C3) (3-5, 8, 10-15). Particles coated with IgG or C3 attach to the corresponding receptors on the plasma membranes of phagocytic cells. We have been studying the roles of these ligands (IgG and C3) and of their recepto...

Moderate hemolytic anemia, abnormal erythrocyte morphology (spherocytosis), and decreased membrane stability are observed in mice with complete deficiency of all erythroid protein 4.1 protein isoforms (4.1(-/-); Shi TS et al. J Clin Invest 103: 331, 1999). We have examined the effects of erythroid protein 4.1 (4.1R) deficiency on erythrocyte cation transport and volume regulation. 4.1(-/-) mice...