PURPOSE Non-small cell lung cancer (NSCLC) with KRAS mutation may be resistant to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI). This study aims to evaluate a plasma-based KRAS mutation analysis and the clinical significance of plasma KRAS mutation as a predictive marker for tumor resistance to EGFR-TKIs in patients with NSCLC. EXPERIMENTAL DESIGN DNA extracted from p...

PIK3CA mutation represents a clinical subset of diverse carcinomas. We explored the status of PIK3CA mutation and evaluated its genetic variability, treatment, and prognosis in patients with lung adenocarcinoma. A total of 810 patients with completely resected lung adenocarcinoma were recruited between 2008 and 2013. The status of PIK3CA mutation and other three genes, that is, EGFR mutation, K...

BACKGROUND Epidermal growth factor receptor (EGFR) mutations play a vital role in the prognosis of patients with lung adenocarcinoma. Such somatic mutations are more common in women who are non-smokers with adenocarcinoma and are of Asian origin. However, to our knowledge, there are few studies that have focused on men. MATERIALS AND METHODS One hundred and eighty-four consecutive patients (9...

PURPOSE KRAS mutations have been used widely as prognostic or predictive marker in patients with advanced colorectal cancer (CRC). However, it may be difficult to obtain a tumor tissue for analyzing the status of KRAS mutation in large proportion of patients with advanced disease. MATERIALS AND METHODS We obtained pairs of tumor and serum samples from 65 patients with advanced CRC, between Ma...

This study aims to evaluate the mutation status of KRAS, BRAF, NRAS, PIK3CA, and AKT analyze association gene mutations with clinicopathological characteristics colorectal cancer. 151 cancer patients were diagnosed at Pathology Molecular Biology Center in National Cancer Hospital K. The research was conducted as a cross-sectional using methods such genomic DNA extraction; analysis by Real-time ...

STUDY DESIGN Population-based longitudinal observational study. OBJECTIVE To describe the prevalence of scoliosis in Rett syndrome, structural characteristics and progression, taking into account the influences of age, genotype, and ambulatory status. SUMMARY OF BACKGROUND DATA Scoliosis is the most common orthopedic comorbidity in Rett syndrome yet very little is known about its natural hi...

BACKGROUND Cigarette smoking is an established risk factor for colorectal cancer. Because colorectal carcinogenesis is a heterogeneous process, we investigated whether cigarette smoking is differentially associated with molecularly defined subtypes of colorectal cancer. METHODS We evaluated associations between smoking and incident colorectal cancer, overall and by microsatellite instability ...

B-RAF is one of the most commonly mutated oncogenes in human cancer. However, the mutation status of B-RAF has not been established completely in HNSCC. We have analysed the mutation status of the kinase domain of the B-RAF gene (exons 11 and 15) in 91 Japanese HNSCC patients as well as 12 HNSCC cell lines. DNA was extracted and amplified by PCR. Mutations were then analysed by SSCP mutation de...