PURPOSE OF REVIEW Though long hypothesized, the putative link between Mycobacterium avium paratuberculosis and Crohn's disease remains neither confirmed nor refuted. This article reviews published contributions that directly or indirectly address this question. RECENT FINDINGS Epidemiologic studies, looking for M. avium paratuberculosis DNA in Crohn's tissue, show a strong association between...

The demand for rapid methods for the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are the most widespread worldwide. Together with the qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. The aim of our study was to compare the results obtained using two different dPCR systems and one qPCR...

Bu çalışmada, önemli ekonomik kayıplara neden olan kronik enfeksiyöz bir hastalık Mycobacterium avium subsp. paratuberculosis (MAP) tarafından oluşturulan paratüberküloz’un Şanlıurfa’daki sığırlarda seroprevalansının belirlenmesi amaçlanmıştır. amaçla, tesadüfi örnekleme ile Şanlıurfa merkez ilçelerinden, sağlıklı 465 sığırdan alınan serumlar kullanıldı. Serum örneklerinden anti-MAP antikorları...

Figure 1. Representative PCR results showing amplification (or otherwise) of 298-bp genomic fragment corresponding to IS900 in diabetic patients (lanes 1–8). Lanes 9 and 10, Control subjects without type 1 diabetes mellitus. Lanes 11 and 12, Mycobacterium avium subspecies paratuberculosis–positive and M. avium subspecies paratuberculosis–negative control subjects, respectively. M, molecular mar...

OBJECTIVE To evaluate the effect of exposure to environmental mycobacteria on results of 2 commercial ELISAs for paratuberculosis in cattle. DESIGN Experimental trial. ANIMALS 19 weaned crossbred beef calves. PROCEDURES Calves were inoculated SC with 1 of 5 mycobacterial isolates (3 calves/isolate) derived from herds with high proportions of false-positive serologic reactions for paratube...

Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating Mycobacterium bovis infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (...

BACKGROUND Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic, wasting, and progressive enteritis in cattle, bringing significant economic losses in livestock industries. MAP has spread worldwide mainly due to movement of animals. The objective of this study was to determine the MAP seroprevalence in cattle in the Xinjiang Uygur Autonomous Region, Northwest China, and evaluate...

Johne's disease (JD) is a chronic enteric infection of cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP). The high economic cost and potential zoonotic threat of JD have driven efforts to develop tools and approaches to effectively manage this disease within livestock herds. Efforts to control JD through traditional animal management practices are complicated by MAP's ability t...

An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimu...