The dehydrogenase activity in mycobacteria is a promising field of research for the following reasons. Youmans et al. (1955, 1957) showed that small particles, containing dehydrogenases and possessing characteristics of mitochondria, stimulate in mice the production of a degree of immunity which is equal to the immunity produced by a 1-mg quantity of whole living cells of H37Ra. On the other ha...

Active transport of proline remained unaffected in phospholipase A-treated electron transport particles from Mycobacterium phlei. However, the steady state level of proline was reduced 50 to 60% in phospholipase A-treated depleted electron transport particles that were devoid of membrane-bound coupling factor-latent ATPase activity. The decrease in the uptake of proline in the phospholipase A-t...

Jin-Feng ZHONG, Xiao-Qing ZHANG, Wei-Gao WU, Wei TU, Zhen-Xiang LIU, Re-Jun FANG* College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China Hunan Co-Innovation Center of Animal Production Safety, Changsha, P.R. China Hunan Polytechnic of Environment and Biology, Hengyang, P.R. China Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohho...

A phospholipase A1 bound tightly to the membranes of Mycobacterium phlei cells was purified approximately 500fold to near homogeneity by extraction with Triton X-100, delipidation with organic solvents, solubilization with sodium dodecyl sulfate, column chromatographies on Sephadex G-200 in the presence of sodium dodecyl sulfate and on DEAE-cellulose in the presence of BRIJ 58, and sodium dodec...

A phospholipase A1 bound tightly to the membranes of Mycobacterium phlei cells was purified approximately 500fold to near homogeneity by extraction with Triton X-100, delipidation with organic solvents, solubilization with sodium dodecyl sulfate, column chromatographies on Sephadex G-200 in the presence of sodium dodecyl sulfate and on DEAE-cellulose in the presence of BRIJ 58, and sodium dodec...

Several years ago experiments on the biochemistry of Drosophila melanogaster (1) directed our attention to a class of substances that are soluble in dry ether but yield a variety of amino acids upon hydrolysis. Moreover, it was observed that in very young larvae essentially all of the non-protein amino acids are found in a lipide-soluble fraction. During subsequent rapid growth and development ...