Introduction Tuberculosis (TB) is the leading cause of death in AIDS patients worldwide; the earliest opportunistic infection occurring in conjunction with HIV; an accelerant of HIV replication and immune system deterioration. Co-infection with Mycobacterium tuberculosis (Mtb) and HIV is an increasing global emergency. Little is known regarding the early events of Mtb infection in humans especi...

Introduction: Mycobacterium tuberculosis is the second most infectious cause of death in adults globally and major health trouble Nepal. The diagnosis pulmonary mainly carried by acid-fast bacilli smear microscopy, culture on Lowenstein–Jensen media, GeneXpert MTB/RIF assay. This study aimed to evaluate Acid-Fast Bacilli stain used for tuberculosis.
 Methods: A cross-sectional was performe...

Purpose] Mycobacterium tuberculosis (MTB) infection should be detected in all patients before progressing to active tuberculosis (TB) ; however, interferon-γ release assays (IGRAs) and serological assays cannot accurately detect TB infection in all patients. Therefore, we conducted a prospective study to determine whether TB infections in patients with active pulmonary TB could be reliably dete...

With 1.6 million casualties annually and 2 billion people being infected, tuberculosis is still one of the most pressing healthcare challenges. Here we report on the new computational docking algorithm FRIGATE which unites continuous local optimization techniques (conjugate gradient method) with an inherently discrete computational approach in forcefield computation, resulting in equal or bette...

BACKGROUND The clinical and pathological features of sarcoidosis and tuberculosis may mimic each other, and when the caseous necrosis is not seen in tuberculosis tissue, differentiation is not easy. OBJECTIVE This study evaluates the ability of real-time PCR quantification and sets the quantitive value to differentiate sarcoidosis from TB. METHODS Formalin-fixed and paraffin-embedded sectio...

We studied the effect of 1,25-dihydroxy vitamin D3 [1,25(OH)2D3] on toll-like receptor (TLR) gene expression in neutrophils cultured with Mycobacterium tuberculosis (Mtb) with or without 1,25(OH)2D3 in pulmonary tuberculosis patients and healthy controls. 1,25(OH)2D3 significantly increased the expression of TLR2, TLR9 and TIRAP genes compared to control cells in both the study groups (P < 0.05...

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) is contracted via aerosol infection, typically affecting the lungs. Mycobacterium bovis bacillus Calmette-Guerin (BCG) is the only licensed vaccine and has variable efficacy in protecting against pulmonary TB. Additionally, chemotherapy is associated with low compliance contributing to development of multidrug-resistant (MDR) and ext...

IFN-γ is a critical mediator of host defense against Mycobacterium tuberculosis (Mtb) infection. Antigen-specific CD4+ T cells have long been regarded as the main producer of IFN-γ in tuberculosis (TB), and CD4+ T cell immunity is the main target of current TB vaccine candidates. However, given the recent failures of such a TB vaccine candidate in clinical trials, strategies to harness CD4-inde...

Tuberculosis (TB) ranks second, next to AIDS making it most formidable disease if the present age. One of the crucial enzymes involved in cell wall synthesis of Mycobacterium tuberculosis, InhA (enoyl acyl carrier protein reductase) has been authenticated as an effective target for anti-mycobacterial drug development. In the current work, we have developed novel derivatives of 1,2,4-triazole-5-...

Previously, we observed that both major membrane protein II of Mycobacterium leprae (MMP-ML) and its fusion with M. bovis BCG (BCG)-derived heat shock protein 70 (HSP70) (Fusion-ML) are immunogenic and that recombinant BCG secreting either of these proteins effectively inhibits the multiplication of M. leprae in mice. Here, we purified M. tuberculosis-derived major membrane protein II (MMP-MTB)...