tuberculosis is one of the most important infectious diseases and a major cause of morbidity and mortality in developing countries. multi drug resistant tuberculosis is a new threat, which can result in a high case fatality rate. this study was conducted to define the incidence of multidrug resistant tuberculosis in kermanshah. ­antibiogram was performed on­­ pcr-confirmed isolates of mycobacte...

Previous studies of antibody responses to Mycobacterium tuberculosis antigens at different stages of tuberculosis (TB) and in different classes of TB patients provided evidence that the profile of antigens recognized by antibodies is altered with disease progression. M. tuberculosis is a slow-growing organism, and it takes several weeks to months for an infection to progress to clinical TB. App...

To better understand the biology and the virulence determinants of the two major mycobacterial human pathogens Mycobacterium tuberculosis and Mycobacterium leprae, their genome sequences have been determined recently. In silico comparisons revealed that among the 1439 genes common to both M. tuberculosis and M. leprae, 219 genes code for proteins that show no similarity with proteins from other...

Lung transplantation is a therapeutic option for the treatment of advanced lung disease. The risk pulmonary infections increased in transplant recipients. We report case coinfection with Mycobacterium tuberculosis and Nocardia species recipient.

A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS6110 and the 65-kDa...

From 1991 to 1996, 541 blood samples were tested for the presence of mycobacteria; 56 were positive (30 patients, 26 human immunodeficiency virus positive). The species found were Mycobacterium avium (41 samples from 18 patients), Mycobacterium tuberculosis (12 samples from 9 patients), and three other species (1 sample each). The average time to detection was 25.23 days (22.65 for M. avium and...

Polypeptide Ags present in the culture filtrate of Mycobacterium tuberculosis were purified and evaluated for their ability to stimulate PBMC from purified protein derivative (PPD)-positive healthy donors. One such Ag, which elicited strong proliferation and IFN-gamma production, was further characterized. The N-terminal amino acid sequence of this polypeptide was determined and used to design ...

A new mycobacteriophage-based technique (PhageTek MB) was compared with standard culture and staining techniques for diagnosis of pulmonary tuberculosis. A total of 2,048 respiratory specimens from 1,466 patients collected from February 2000 to March 2001 were studied by both (i) conventional methods (direct microscopic examination [auramine-rhodamine fluorochrome], and culture in BacT/ALERT 3D...