Background Mycoplasma testing on cell lines or biological products used to be performed based on classical methods such as agar and broth medium and/or indicator cell culture. However, these methods require a long incubation period and are not adapted for samples, like live-attenuated vaccine viruses (which can not completely be neutralized) or cell therapy products (with short shelf life). NAT...

OBJECTIVE This study was undertaken to assess the impact of mycoplasma strains (Mycoplasma hominis or Ureaplasma urealyticum) on the development of chronic endometritis. METHODS Fifty-eight patients with acute pelvic infection were enrolled in this prospective cohort study. Endometrial cultures and biopsies were obtained on admission and 5-7 and 21-28 days after completion of treatment. RES...

The aim of the present study was to investigate the relationships between serum amino acid profiles in normal and calves with Mycoplasma bronchopneumonia. Serum free amino acid concentrations in serum obtained from 34 calves with or without Mycoplasma bronchopneumonia were determined by high-performance liquid chromatography. The calves with Mycoplasma were characterized by significantly lower ...

Because mycoplasmas may be a cofactor in the progression of human immunodeficiency virus infection to AIDS, their susceptibilities to antibiotics need to be known in the event that appropriate therapy is required. The mycoplasmas studied were a stock culture strain of Mycoplasma fermentans, two strains of M. fermentans isolated from patients with AIDS, M. fermentans var. incognitus, Mycoplasma ...

Four agar media (PPLO, NYC, A7B, and E) which are commonly used for the isolation of urogenital tract Mycoplasma species and Ureaplasma urealyticum were compared by culturing swabs of the endocervix of 334 pregnant women on all four media. To permit growth of both Mycoplasma and U. urealyticum, selective ingredients were omitted from the media tested. A7B and E agar were both satisfactory for t...

Recent studies have been conducted in Brazil using molecular techniques for the detection of hemotrophic mycoplasmas in several mammals. In domestic cats, Mycoplasma haemofelis, "Candidatus M. haemominutum", and "Candidatus M. turicensis" infections have been identified. These species have also been found in free-ranging and captive neotropical felid species. Two canine hemoplasmas, Mycoplasma ...

BACKGROUND Mycoplasma is a common cause of respiratory infections and may require differential diagnosis from Kawasaki disease (KD). In this study, we investigated the frequency and clinical manifestations of mycoplasma infection in patients with KD. MATERIALS AND METHODS Medical records of 375 in-patients admitted for treatment during the acute stage of KD, were collected, and reviewed retro...

The mean flock size was ten chickens per rural farmer. Antibodies to Mycoplasma gallisepticum and Mycoplasma synoviae were detected in 57.88% and 67.33% of the chicken sera respectively.

Since the isolation by Dienes and Esdall (1937) of a strain of Mycoplasma from a patient with a Bartholin's gland abscess, numerous attempts have been made to provide definite proof of the aetiological role of Mycoplasma in urogenital tract infections, especially nongonococcal urethritis (NGU). Most of the earlier studies were concerned with Mycoplasma hominis, but it now appears that this spec...

Mycoplasma contamination is a common problem with continuously cultured cell lines. Contamination, which may go undetected for long periods of time, can alter cellular proliferation, gene expression, and other cellular responses. Early detection of this insidious pest is an important part of good tissue culture quality control. Here we describe the detection of Mycoplasma using the SynergyTM 2 ...