Two nicotinamide adenine dinucleotide (NAD(+)) analogues modified at the 6 position of the purine ring were synthesized, and their substrate properties toward Aplysia californica ADP-ribosyl cyclase were investigated. 6-N-Methyl NAD(+) (6-N-methyl nicotinamide adenosine 5'-dinucleotide 10) hydrolyzes to give the linear 6-N-methyl ADPR (adenosine 5'-diphosphoribose, 11), whereas 6-thio NHD(+) (n...

Life or death in hostile environments depends crucially on one's ability to detect and gate novel sounds to awareness, such as that of a twig cracking under the paw of a stalking predator in a noisy jungle. Two distinct auditory cortex processes have been thought to underlie this phenomenon: (i) attenuation of the so-called N1 response with repeated stimulation and (ii) elicitation of a mismatc...

Objective. We studied circulating precursor cells (CPC) in type 2 diabetes mellitus (T2DM) with neuropathic foot lesions with or without critical limb ischemia and relationships between endothelial precursor cells (EPC) and peripheral neuropathy. Methods and Subjects. We measured peripheral blood CD34, CD133, and CD45 markers for CPC and KDR, CD31 markers for EPC by citofluorimetry and systemic...

The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood an...

The structure of multivariate hypergeometric terms is studied. This leads to a proof of (a special case of) a conjecture formulated by Wilf and Zeilberger in 1992. A function u(n1, . . . , nd) with values in a field K is called a hypergeometric term if there exist rational functions Ri ∈ K(n1, . . . , nd), i = 1, . . . , d, such that u is solution of a system of d firstorder recurrences Si · u ...

Auditory evoked potential (AEP) correlates of the neural representation of stimuli along a /ga/-/ka/ and a /ba/-/pa/ continuum were examined to determine whether the voice-onset time (VOT)-related change in the N1 onset response from a single to double-peaked component is a reliable indicator of the perception of voiced and voiceless sounds. Behavioral identification results from ten subjects r...

In this research, a Brønsted acidic ionic liquid namely N1,N1,N2,N2-tetramethyl-N1,N2-bis(sulfo)ethane-1,2-diaminium chloride {[TMBSED][Cl]2} was employed as a highly efficient catalyst for the solvent-free production of some nitrogen- and oxygen-containing heterocyclic compounds including: (i) polyhydroquinolines (from arylaldehydes, dimedone, β-ketoesters and ammonium acetate), (ii) 3,4-dihyd...

N1,N1,N2,N2-tetramethyl-N1,N2-bis(sulfo)ethane-1,2-diaminium chloride ([TMBSED][Cl]2) was synthesized as an acidic ionic liquid and characterized using FT-IR, 1H and 13C NMR, mass spectroscopy, TG, DTG and DTA techniques. This ionic liquid was employed as efficient catalyst for the extremely facile and efficient synthesis of dihydropyrrol-2-ones and functionalized tetrahydropyridines. One-pot f...

conclusions: the recombinant baculovirus constructed in this work has proper characteristics to produce h5n1 influenza virus-like particles in sf9 cells. results: restriction map of pfastbacihnm1 plasmid confirmed the fidelity of the clone. the pcr carried out on the recombinant bacmids as template indicated that a proper homologous recombination has occurred between pfastbacihnm1 donor plasmid...