background and objectives: hbha and mtb32c have been isolated from culture supernatants of mycobacterium tu- berculosis (m. tuberculosis) and mycobacterium bovis (m. bovis) and their immunogenicity previously studies have been confirmed. in this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (mt- b32c-hbha), in producing new chimeric protein under t...

angiocardiography is an x-ray examination of the blood vessels or chambers of the heart. cardiologists and staff members applying this procedure are exposed to high levels of scattered radiation. in our previous study the incidence of unstable chromosomal aberrations and cytokinesis-blocked micronuclei were found to be significantly higher in exposed individuals than the age and sex matched con...

introduction: satellite cells are known as the main regenerative cell type in skeletal muscles. our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. me...

background: diabetes is a major public health problem. the development of new therapies that are able to improve glycemia management and even to cure diabetes is of great interest. in this study, protective effects of sodium tungstate against stz-induced beta-cell damages were investigated. methods: sixty rats were divided into six groups: control, diabetic, sodium tungstate treated diabetic ra...

background: pectic acid extracted from plants increases the secretion of prolactin (prl) when injected intravenously into ewes or fed to rats. fragments of ewe hypophysis and lactating rabbit mammary gland incubated in vitro in the presence of pectic acid secreted more prl and caseins compared to the controls. however, it is not known whether pectic acid directly stimulates prl secretion in pit...

stem cell research has obtained much prominence in recent years for its therapeutic potential in dealing with serious diseases, many of which are essentially incurable by routine therapies. mesenchymal stem cells with pluripotency and immunomodulatory properties are suitable candidates for tissue engineering and regenerative medicine. today, nanofibrous scaffolds are widely used in tissue engin...

introduction: d-galactose (d-gal) is well known as an appropriate agent to induced aging effects in the in vivo and in vitro models. in the present study, we selected crocin, the main constituent of crocus sativus l. (saffron), against d-gal cytotoxicity in human neuroblastoma sh-sy5y cells. materials and methods:  cells were pretreated with crocin (25-500 µm) for 24 h and then exposed to d-gal...

objective(s): to investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. in addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (tips) using in silico studies. materials and methods: human adipose derived stem cells (hascs) were studied under treatments with 2.5 µm/ml cinnamaldehyde, 0....

dead yeast cells are an abundant residue of the brazilian ethanol industry and the industries still don’t have a proper or desirable destiny for all these cells, which means tons of vitamins and proteins that have not a major end. in the other hand, heavy metal residues are a problem, especially for leather industry, where brazil is also a great producer. in this work, dead yeast cells were us...

it is well known that several plant extracts have a lactogenic effect. this response may be inducedthrough the stimulation of prolactin release from lactotrope cells. a partial purification of the activecompound indicated that it is rich in pectin and β-glucan. these materials stimulate secretion of prolactinfrom hypophyseal fragments. in the present study, the effect of β-glucan on the secreti...