Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis, a suspect causative agent of Crohn's disease in man, is an emerging disease of international proportions affecting all ruminants. Early stage detection of Mycobacterium avium subsp. paratuberculosis infection would accelerate progress in control programmes. Despite new molecular approaches the standard diagnostic test for ...

background :  q fever is an important widespread reemerging zoonosis. the presence of coxiella burnetii in 100 tick-infested dogs was assessed in this study. methods :  the blood samples from 100 referred dogs were acquired and evaluated by nested-pcr. results :   c. burnetii was detected in 11 out of 100 (11%) blood samples. most of the positive dogs were kept outdoor and fed on raw diet. base...

background: cutaneous leishmaniasis is an annoying and disfiguring disease affecting around 1,500,000 individuals globally. there are endemic pockets of this disease in taif region. in some patients, lesion often weeps and leads to scar formation. the study was conducted to see the efficacy of fluconazole and itraconazole in the patients of cutaneous leishmaniasis and the effect of these drugs ...

background: parasitological methods for the diagnosis of visceral leishmania-sis (vl) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. we evaluated a loop mediated isothermal amplification (lamp) assay using blood from vl pa-tients and compared it to nested pcr. methods: forty-seven subjects with clinical fea...

background: wolbachia  are common intracellular bacteria that infect different groups of arthropods including mos­quitoes. these bacteria modify host biology and may induce feminization, parthenogenesis, male killing and cyto­plasmic incompatibility (ci). recently wolbachia  is being nominated as a bio-agent and paratransgenic candidate to control mosquito borne diseases. methods: here we repor...

background and objective : given the importance of rapid diagnosis for fungal rhinosinusitis, this study aimed to evaluate the use of nested pcr to identify aspergillus and mucor species in clinical samples from patients with suspected fungal rhinosinusitis. methods : functional endoscopic sinus surgery specimens were collected from 98 patients with rhinosinusitis from 2012 to 2013. all samples...

background: chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. early detection and treatment of c.trachomatis genital infection prevent serious reproductive complications.objective: performances of enzyme immunoassay (eia) and major outer membrane protein (momp)-polymerase chain reaction (pcr) for diagnosis of genital c.trachomatis infection in women wer...