Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behav...

Cell type-specific transcriptomes are enabled by the action of multiple regulators, which are frequently expressed within restricted tissue regions. In the present study, we identify one such regulator, Quaking 5 (Qki5), as an RNA-binding protein (RNABP) that is expressed in early embryonic neural stem cells and subsequently down-regulated during neurogenesis. mRNA sequencing analysis in neural...

Neural stem cells reside in a specialized vascular microenvironment that is termed “neural stem cell niche”, Brain vessels are the most important element in the NSC niche and paly an important role in balancing NSC’s maintenance and differentiation in order to maintain tissue homeostasis. In this study, we developed reconstituted 3D in vitro neural stem cell-vascular niche that consists of extr...

Ceramide and its derivative sphingosine-1-phosphate (S1P) are important signaling sphingolipids for neural stem cell apoptosis and differentiation. Most recently, our group has shown that novel ceramide analogs can be used to eliminate teratoma (stem cell tumor)-forming cells from a neural stem cell graft. In new studies, we found that S1P promotes survival of specific neural precursor cells th...

We have characterized the profile of membrane currents in an immortalized human neural stem cell line, HB1.F3 cells, using whole-cell patch clamp technique. Human neural stem cell line generated from primary cell cultures of embryonic human telencephalon using a replication-incompetent retroviral vector containing v-myc expresses nestin, a cell type-specific marker for neural stem cells. The hu...

Introduction: Embryonic Stem (ES) cells as pluripotent cells derived from the inner cell mass of blastula can differentiate to neural cells in vitro and this property is valuable in studies of neurogenesis and in the generation of donor cells for transplantation. In this regard, the propose of this research, was the study of the role of two important factors in the development of neural syst...

background: bone marrow stromal stem cells (bmsc) are appropriate source of multipotent stem cells that are ideally suited for use in various cell-based therapies. it can be differentiated into neuronal-like cells under appropriate conditions. this study examined the effectiveness of co-stimulation of creatine and retinoic acid in increasing the differentiation of bmsc into gabaergic neuron-lik...

traumatic brain injury (tbi) is described by a disruption in the normal function of the brain due to an injury following a trauma, which can potentially cause severe physical, cognitive, and emotional impairment. the use of human stem cells and self-assembling peptide scaffolds suggest huge potential for application in the treatment of tbi. in the present study, we surveyed the beneficial effec...

in this center, from 1991 to 2002, 89 chronic myelogenous leukemic (cml) patients, age ranging between 8-48 years with a median age of 29, underwent hematopoietic stem cell transplantation. eighty-eight patients were in the first chronic phase of disease. twenty-three patients received bone marrow transplantation (bmt) and 66 patients received peripheral blood stem cell transplantations (pbsct)...