In previous publications (4, 6), the preparation and properties of a particulate enzyme complex from Rihizobiumn japonicutm cells isolated from the nodules of soybean plants (Glycine max Merr.) have been described. It has been established that the particles catalyze the reduction of nitrate to nitrite and that either DPNH or succinate serves as effective electron donor. Evidence indicates that ...

A 4.0-kb DNA fragment of Trichodesmium sp. strain WH9601 contained gene sequences encoding the nitrate reduction enzymes, nirA and narB. A third gene positioned between nirA and narB encodes a putative membrane protein with similarity to the nitrate permeases of Bacillus subtilis (NasA) and Emericella nidulans (CrnA). The gene was shown to functionally complement a DeltanasA mutant of B. subtil...

Two polytopic membrane proteins, NarK and NarU, are involved in nitrate and nitrite uptake and nitrite extrusion by Escherichia coli. A third polytopic membrane protein, NirC, functions only in nitrite transport. During exponential growth, the quantity of NarU in membrane fractions was <0.01% of the quantity of NarK. During the stationary phase of growth, the ratio of NarU to NarK increased to ...

UNLABELLED Single dose administration of dietary inorganic nitrate acutely reduces blood pressure (BP) in normotensive healthy volunteers, via bioconversion to the vasodilator nitric oxide. We assessed whether dietary nitrate might provide sustained BP lowering in patients with hypertension. We randomly assigned 68 patients with hypertension in a double-blind, placebo-controlled clinical trial ...

pipe drain installation depth is one of the most important design parameters, which has a key role in the drained water volume and quality. in this research, the effect of pipe drain installation depth was investigated by using a physical laboratory model. drains were installed at 0.25, 0.5 and 0.85 m deep in a cubical box with dimensions of 1*0.5*1 meter filled with a sandy loam soil. nitrate,...

Nitrate reductase of the salt-tolerant alga Dunaliella parva could utilize NADPH as well as NADH as an electron donor. The two pyridine nucleotide-dependent activities could not be separated by either ion exchange chromatography on DEAE-cellulose or gel filtration on Sepharose 4B. The NADPH-dependent activity was not inhibited by phosphatase inhibitors. NADPH was not hydrolyzed to NADH and inor...