Abstract The decolourization of an aqueous solution the textile dye Acid Red 88 (AR88) and control invasive plant species Acacia dealbata Link . (ADL) were addressed in this work. aims study (1) characterization ADL pollen, (2) application pollen powder adsorption processes, (3) selection best operational conditions for nitriloacetic acid (NTA)-UV-A-Fenton process (4) assess efficiency combined...

The inactivation of the Zn+2 metallo enzyme acylamino acid amido hydrolase from Aspergillus oryzae by ethylendiamine-tetraacetate (EDTA) and nitrilotriacetate (NTA) and the effects of Phe and His on this process were studied. Reactivation of the enzyme by Zn+2or Co+2-NTA buffer revealed a dissociation constant for the Zn+2-enzyme of 10~10 M and for the Co+2-enzyme of 10-7-5 m. The kinetic prope...

Abbreviations: CAPS: N-cyclohexyl-3-aminopropanesulfonic acid cDNA: omplementary DNA to mRNA CHES: N-cyclohexyl-2-aminoethanesulfonic acid. DTT: dithiothreitol FPLC: fast protein liquid chromatography IPTG: isopropylthio-β-D-galactoside LB: Luria-Bertani MES: 2-(N-morpholino)ethanesulfonic acid Ni-NTA: Nickel-nitriloacetic acid ORF: open reading frame PCR: polymerase chain reaction PMSF: phenyl...

Porphyromonas gingivalis, a periodontal pathogen, has the ability to lyse erythrocytes. The hemolytic activity of P. gingivalis A7436 was purified as a 45-kDa protein from the culture supernatant of a 3-days old culture using nickel-nitrilotriacetic acid chromatography. Erythrocytes treated with purified P. gingivalis hemolysin showed the presence of pores and extracellular debris by scanning e...

The first and main part of this thesis is focused on the design and synthesis of photo-activable and metal chelating alkanethiols. Chelate associated photochemistry (CAP) is a novel concept of combining two well-known protein (ligand) immobilization strategies to obtain a sensor surface of covalently bound ligand with defined orientation. This includes nitrilotriacetic acid (NTA) which is used ...

conclusions results showed that hcvcp + bcg induced a moderate ctl and mixed th1/th2 immune responses with higher levels of cell proliferation and ifn-γ secretion, indicating that bcg may have a better outcome when formulated in hcvcp-based subunit vaccines. results expression and purification of core protein around the expected size (21 kda) was confirmed by western blotting. the hcvcp + bcg v...

A two-step templated, ribosomal biosynthesis/printing method for the fabrication of protein microarrays for surface plasmon resonance imaging (SPRI) measurements is demonstrated. In the first step, a sixteen component microarray of proteins is created in microwells by cell free on chip protein synthesis; each microwell contains both an in vitro transcription and translation (IVTT) solution and ...

BACKGROUND & OBJECTIVE Anthrax has been reported from almost every country and India is endemic for this disease. There is considerable under reporting of the disease because of lack of microbiological facilities and diagnostic reagents. In India only conventional methods which have limitations, are being used to diagnose the disease. Hence the aim of this study was to isolate and purify protec...

We have examined the effects of aqueous complexation on rates of dissimilatory reductive precipitation of uranium by Shewanella putrefaciens. Uranium~VI! was supplied as sole terminal electron acceptor to Shewanella putrefaciens ~strain 200R! in defined laboratory media under strictly anaerobic conditions. Media were amended with different multidentate organic acids, and experiments were perfor...