rotavirus nonstructural glycoprotein nsp4 can induce diarrhea in newborn mice. it has been suggested that nsp4 may be a key determinant for rotavirus pathogenesis and a target for vaccine development. in order to study the biological and morphological role of nsp4 a large amount of the purified protein and antibody against it are required. simian rotavirus sa11 was propagated in bscl cell, puri...

We report here the complete genome sequence of Mayaro virus strain BeAr 20290 isolated from Haemagogus mosquitoes in 1960. The sequence presented here includes all nonstructural and structural proteins and the 5'- and 3'-untranslated (UTR) regions.

A second nonstructural protein of the Aleutian disease parvovirus was predicted from nucleotide sequence analysis and a detailed transcription map. Western immunoblotting analysis showed that infected mink and ferrets show an antibody response to this predicted protein.

In aWestNile virus (WNV)-free ecosystem, it is essential to verify the integrity ofRNA before concluding thatRNAextracted frommosquito specimens is negative forWNVgene sequences. Theprimaryobjectiveofour studywas todevelopa rapidmolecular assay to rapidly screenmosquitoes for the presence of 18S RNA andWNV gene sequences. Mosquitoes, collected frommultiple sites on the island of OÔahu, were poo...

The nucleotide sequences of RNA segment 7 (nonstructural protein gene; NS) were compared among 34 influenza C virus strains isolated between 1947 and 1992. The results showed that all the NS genes analysed had the potential to encode NS1 and NS2 proteins of 246 and 182 amino acids, respectively. The deduced amino acid sequence of the previously unidentified NS2 was fairly well conserved, althou...

The Cornell Net Carbohydrate and Protein System (CNCPS) has a submodel that predicts rates of feedstuff degradation in the rumen, the passage of undegraded feed to the lower gut, and the amount of ME and protein that is available to the animal. In the CNCPS, structural carbohydrate (SC) and nonstructural carbohydrate (NSC) are estimated from sequential NDF analyses of the feed. Data from the li...