The electron shuttle heme protein Cyt-c(6) from the photosynthetic cyanobacterium Nostoc sp. PCC 7119 was immobilized on nanostructured Ag electrodes coated with SAMs that mimic different possible interactions with its natural reaction partner PSI. The structure, redox potential, and electron-transfer dynamics of the SAM-Cyt-c(6) complexes were investigated by TR-SERR spectroelectrochemistry. I...

Title 1 Identification of Homophenylalanine Biosynthetic Genes from Cyanobacterium Nostoc 2 punctiforme PCC73102 and Its Application to Microbial Production by Escherichia coli 3 4 Running title 5 Identification of homophenylalanine biosynthetic genes 6 7 Kento Koketsu, Satoshi Mitsuhashi, Kazuhiko Tabata 8 Bioprocess Development Center, Kyowa Hakko Bio Co., Ltd., Tsukuba, Ibaraki, Japan 9 10 C...

Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect...

The effect of boron on heterocystous and nonheterocystous dinitrogen fixing Cyanobacteria was examined. The absence of boron in culture media inhibited growth and nitrogenase activity in Nodularia sp., Chlorogloeopsis sp., and Nostoc sp. cultures. Examinations of boron-deficient cultures showed changes in heterocyst morphology. However, cultures of nonheterocystous Cyanobacteria, Gloeothece sp....

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE possesses robust trans-splicing activity with an efficiency of > 98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 (Ssp DnaE). Both the N- and C-terminal parts of the split Npu D...

Using a pure, bacteria-free culture of Nostoc muscorum as a bioassay test organism, it was found that the water of Bass Lake, Livingston Co., Michigan, had a severe phosphate deficiency and less severe deficiencies of K, Fe, Mn, and Mg. Emission spectrographic analyses showed that the concretions had Ca as the principal constituent, Mg as the major impurity, and Fe, Si, Mn, Al, and Sr as minor ...