نتایج جستجو برای: nucleic acid amplification
تعداد نتایج: 804699 فیلتر نتایج به سال:
Nucleic acid sequence-based amplification (NASBA) is an isothermal technique that allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A rapid and specific NASBA technique was developed, allowing the detection of foot-and-mouth disease virus genetic material in a range of sample mater...
Since it is not possible to state with certainty that contamination has occurred during a nucleic acid amplification assay in the absence of a positive result for a negative control, methods of elementary probability theory are used to illustrate how to identify those runs in which the possibility of contamination should be considered. The use of binomial and Poisson distributions and an analys...
BACKGROUND Global analysis of the genome, transcriptome, and proteome is facilitated by the recent development of tools for large-scale, highly parallel analysis. We describe a novel nucleic acid amplification system that generates products by several methods. 3'-Ribo-SPIA primes cDNA synthesis at the 3' polyA tail, and whole transcript (WT)-Ribo-SPIA primes cDNA synthesis across the full lengt...
cytotoxicity evaluation of daphne mucronata (thymelaeaceae) extract and one of its active purified components, using seven different cancerous cell lines, revealed the strong antiproliferative activity of the plant. using flow cytometry technique, it was found that treatment of the most responsive cells (k562) with the plant extract or the active component inhibited the progression of cells thr...
A Syringe-Based and Centrifugation-Free DNA Extraction Procedure for the Rapid Detection of Bacteria
Several bacteria are known to cause food poisoning; therefore, diagnostic systems that detect essential. Nucleic acid-based testing methods involve polymerase chain reaction (PCR) amplification of great interest due their high sensitivity and specificity. Herein, we developed a syringe-based one-step DNA extraction device streamlines the genomic (gDNA) from within 2 min, enabling versatile appl...
COVID-19, the infectious pandemic disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This deadly was unknown before its catastrophic outbreak of infection in Wuhan city China, December 2019. The situation has increased demand rapid enhancement in-vitro diagnostic assays which would enable mass screening and testing. Several molecular serological diagnostics such ...
Real-time reverse transcription-polymerase chain reaction (RT-PCR) is currently considered the most sensitive method to study low abundance gene expression. Since comparison of gene expression levels in various tissues is often the purpose of an experiment, we studied a tissue-linked effect on nucleic acid amplification. Based on the raw data generated by a LightCycler instrument, we propose a ...
To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nuclei...
We collected specimens from women who tested positive for Neisseria gonorrhoeae using the Gen-Probe APTIMA Combo 2 (AC2), and we performed confirmatory tests using a nucleic acid amplification test (NAAT) that targets alternate gonococcal nucleic acid sequences. Among 59,664 specimens, 280 (0.47%) had positive results using AC2; 265 of these specimens were tested using the confirmatory NAAT, of...
Demand for high quality gene expression data has driven the development of revolutionary microarray technologies. The quality of the data is affected by the performance of the microarray platform as well as how the nucleic acid targets are prepared. The most common method for target nucleic acid preparation includes in vitro transcription amplification of the sample RNA. Although this method re...
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