background: the aim of this study was to evaluate the efficacy of arterial blood gases (abgs) in determining the type of intoxication. methods: medical charts of all patients who had referred to the toxicology ward of baharloo hospital between january 2009 and december 2009 were evaluated. after excluding the patients with multi-drug toxicity, data, including age, sex, type of intoxication (the...

DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particul...

The NUVIEW software package allows skeletal models of any double helical nucleic acid molecule to be displayed on a graphics monitor and to apply various rotations, translations and scaling transformations interactively, through the keyboard. The skeletal model is generated by connecting any pair of representative points, one from each of the bases in the basepair. In addition to the above ment...

PNA is a nucleic acid analog with an achiral polyamide backbone consisting of N-(2-aminoethyl)glycine units (figure 1). The purine or pyrimidine bases are linked to the each unit via a methylene carbonyl linker (1-3) to target the complementary nucleic acid (4). PNA binds to complementary RNA or DNA in a parallel or antiparallel orientation following the Watson-Crick base-pairing rules (5-7). T...

Dimethylnitrosamine is metabolized to form an alkylating intermediate, which may have significance for its carcinogenic action. However, certain other compounds that are known to be highly mutagenic, including nitrous acid and hydroxylamine, might also be formed. Owing to the general reactivity of these compounds, it would be difficult to detect their formation in the intact animal. Instead, th...

Guanosine forms hydrogen-bonded dimers in DMSO. The equilibrium constant for dimer formation is 0.18 l./mol at 32”, and there is no evidence of higher order aggregates. The structure of the dimer is probably similar to the hydrogen bonding thought to occur in guanylic acid gels but it is not possible from our results to assign a unique hydrogen-bonding scheme. Cytidine and 1-methylguanosine sho...

Viruses are the most common causes of aseptic meningitis. Early detection, treatment and management of viral meningitis are priority. This study aimed to evaluate common viral meningitis in children referred to Taleghani pediatrics hospital in Gorgan, south east of Caspian Sea, Iran. In this descriptive study CSF and blood samples were taken from 40 children with negative bacterial culture who ...

Most nucleic acid-based technologies rely upon sequence recognition between an oligonucleotide and its nucleic acid target. With the aim of improving hybridization by decreasing electrostatic repulsions between the negatively charged strands, novel modified oligonucleotides named Zip nucleic acids (ZNAs) were recently developed. ZNAs are oligonucleotide-oligocation conjugates whose global charg...