Pseudomonas savastanoi pv. causes olive knot disease that afflicts trees globally. A sensitive and reproducible in vitro pathogenicity bioassay using detached leaf segments was developed after isolation of Psav on KB medium amended with vancomycin, cephalexin cycloheximide. Symptoms were clear under 20X magnification 10 days. This will facilitate rapid screening germplasm collection for resista...
