OBJECTIVE To estimate age-specific probabilities of live birth with oocyte cryopreservation in nondonor (ND) egg cycles. DESIGN Individual patient data meta-analysis. SETTING Assisted reproduction centers. PATIENT(S) Infertile patients undergoing ND mature oocyte cryopreservation. INTERVENTION(S) PubMed was searched for clinical studies on oocyte cryopreservation from January 1996 throu...

Oocyte maturation is critical for successful fertilization and pregnancy. The mechanism underlying the control of this complex process remain relatively unknown, and even more so the specific mechanism through which oocytes acquire competentce to restrain apoptosis and resume meiosis. Insulin-like Growth Factor 1 (IGF-1) is a growth factor that had been frequently used in inducing oocyte matura...

Histological analysis was carried out on ovarian development in the picnic seabream, Acanthopagrus berda (Forsskål 1775) caught estuarine waters of Calicut, Kerala, India from January to December 2016. Four developmental phases oocyte were identified viz., oogonia proliferation and transformation into primary oocyte, growth phase, secondary phase maturation these are similar those described for...

After artificial insemination and multiple ovulation and embryo transfer (MOET), in vitro production of embryos (IVP) represents the third generation of techniques aimed at a better control of animal reproduction. This technique involves four major steps (Figure 1): oocyte collection, oocyte in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro development of the resulting embryo...

Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses...

MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine 1 cumulus cells 2 Bo Pan, Derek Toms, Wei Shen, Julang Li 3 Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario 4 Laboratory of Germ Cell Biology, College of Animal Science and Technology, Qingdao 5 Agricultural University, Qingdao, China 6 7 Abbreviated title: Cumulus cell miR-378 i...

Objective The measurement of follicular output rate (FORT) has been proposed as a good indicator for evaluating follicular response to the exogenous recombinant folliclestimulating hormone (rFSH). This places FORT as a promising qualitative marker for ovarian function. The objective of the study was to determine FORT as a predictor of oocyte competence, embryo quality and clinical pregnancy aft...

While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectivel...

Intracellular Ca(2+) oscillations at fertilization are responsible for triggering egg activation. The aim of this study was to examine the effect of the age of the oocyte donor and in-vitro maturation on the generation of Ca(2+) transients at fertilization. The results show that <10% of in-vivo and in-vitro matured oocytes from 19-day old mice develop to the blastocyst stage in vitro. In contra...