We used single-channel kinetic analysis to study the inhibitory effects of tacrine on human adult nicotinic receptors (nAChRs) transiently expressed in HEK 293 cells. Single channel recording from cell-attached patches revealed concentration- and voltage-dependent decreases in mean channel open probability produced by tacrine (IC(50) 4.6 microM at -70 mV, 1.6 microM at -150 mV). Two main effect...

To establish the relationship between cholinergic ligand occupancy and channel open states, we recorded single-channel currents activated by different acetylcholine (ACh) concentrations from Torpedo californica ACh receptors reconstituted in lipid bilayers. Inspection of single-channel records shows that the frequency of occurrence of long openings increases with ACh concentration. Analysis of ...

Electrical Resistance tomography (ERT) has been previously employed to measure sediment concentrations in liquids (Bolton et al. [1], James et al. [2], Qiu et al. [3]), and linear sensors were the first type of sensor employed in previous tomographic applications (Qiu et al. [4]). However, ERT has mostly been used in cylindrical vessels or pipes with a circular sensor around the area of interes...