نتایج جستجو برای: open reading frame p orf p
تعداد نتایج: 1777068 فیلتر نتایج به سال:
The single-stranded, positive-sense and tripartite RNA virus Cucumber mosaic virus (CMV) was used in this study as a method for monitoring the initial stages of virus infection following aphid transmission. The RNA2 of CMV was modified to incorporate, in a variety of arrangements, an open reading frame (ORF) encoding an enhanced green fluorescent protein (eGFP). The phenotypes of five engineere...
No effective recombinant vaccines are currently available for any rickettsial diseases. In this regard the first non-ribosomal DNA sequences from the obligate intracellular pathogen Piscirickettsia salmonis are presented. Genomic DNA isolated from Percoll density gradient purified P. salmonis, was used to construct an expression library in lambda ZAP II. In the absence of preexisting DNA sequen...
The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode...
Abamectin has been used to control the diamondback moth, Plutella xylostella (P. xylostella), which is a major agricultural pest that can rapidly develop resistance against insecticides including abamectin. Although cytochrome P450 has been confirmed to play an important role in resistance in P. xylostella, the specific P450 genes associated with the resistance are unclear. The full-length cDNA...
Phytophthora species are phytopathogenic oomycetes that damage a wide variety of crops. delivers effectors, which secretory proteins, into the host cells. Effectors promote infection by reprogramming cellular machinery and key determinants oomycete virulence. The major class effector proteins contains RXLR motif. In this study, we have carried out molecular structural characterisation an (RXLR6...
Two species of genus Pueraria ((Pueraria lobata (synonym: montana var. lobata) and mirifica candollei mirifica)) are traditional plants used in medicine since ancient times. These have been became commercially crucial indigenous medicinal plants. Currently, both roots flowers P. as a dietary supplement functional food for women because their rich source phytoestrogen nutrition. However, little ...
Peroxidases are involved in many plant processes including plant defense responses to biotic and abiotic stresses. We isolated a novel peroxidase gene CanPOD from leaves of pepper cultivar A3. The full-length gene has a 1353-bp cDNA sequence and contains an open reading frame (ORF) of 975-bp, which encodes a putative polypeptide of 324 amino acids with a theoretical protein size of 34.93 kDa. ...
The Onsager reaction-field (ORF) theory is extended to apply to three-dimensional Bravais lattices with a basis. The ORF calculation is used to predict the critical temperature for classical Ising, XY, and Heisenberg magnetic models, in particular, on diamond and hexagonal close packed lattices. Results are compared with series extrapolations and other theoretical approaches where available. Fo...
Analysis of porcine transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) mRNA species indicated a deletion in mRNA 3 of PRCV. Polymerase chain reaction (PCR) was used to clone the 5' end of mRNA 3 from PRCV for comparison with the equivalent region in TGEV. Small deletions were observed within and around the PRCV sequence equivalent to the putative open reading ...
Large collections of protein-encoding open reading frames (ORFs) established in a versatile recombination-based cloning system have been instrumental to study protein functions in high-throughput assays. Such 'ORFeome' resources have been developed for several organisms but in virology, plasmid collections covering a significant fraction of the virosphere are still needed. In this perspective, ...
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