1. Pneumococci contain an intracellular enzyme of marked lipolytic activity as measured by the acid liberated by its action on tributyrin. 2. Enzyme-containing solutions may be prepared by dissolving pneumococci in bile, or by extraction by other means. 3. The optimum reaction for maximum activity of the endolipase is about pH 7.8, which coincides with the optimum hydrogen ion concentration for...

Chitooligosaccharides could be produced from chitosan by a newly isolated microorganism, Bacillus thuringiensis ZJOU-010, which exhibited chitosanase activity using shrimp shell wastes as the carbon/nitrogen source. In this study, a 2 central composite experimental design was performed to improve the chitosanase activity by response surface methodology (RSM). The mathematical model developed wa...

background: enzymes are well known as sensitive catalysts in the laboratory and industrial scale. to improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. enzymes immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve ...

Three experiments (Exp.) were conducted to determine the interactive effects of sodium bentonite (NaB) with the efficacy of monensin (MON) or salinomycin (SAL) in coccidiosis-infected chicks. Male broiler chicks 5 to 14 d of age were used, and each treatment was replicated with eight (Exp. 1) or four (Exp. 2 and 3) pens of five chicks each. In Exp. 1, MON (80 ppm), NaB (0.50%), or MON+NaB were ...

Mitochondrial fractions isolated from thoracic muscles of the tobacco horn worm larvae and moths, free of bacterial contaminations, incorporate amino acids into proteins. Optimum activity requires the presence of ATP, an ATPgenerating system, and an amino acid mixture. The pH optimum is around 7.2. The system is not affected by cycloheximide, ribonuclease, or actinomycin D. Puromycin completely...

Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, resp...

The investigation was directed towards the effects of reaction conditions, substrates and pH on the carboxylation reaction of ribulose-1 ,5-bisphosphate carboxylase-oxygenase in the crude enzyme extracts from several plants. Optimal substrate concentrations (H CO j and RubP) were determined. The highest carboxylase activity was attained with Tris/HCl buffer. The pH activity profile was quite sh...