Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic competitive immune assay (EMIT 2000). There was ...

We have used an in vitro system to study the effects of major histocompatibility complex class I binding peptides on thymic development. Fetal thymus lobes from mice deficient in the class I light chain (beta 2 microglobulin or beta 2 M-/-) were cultured for 10 d in vitro, during which time T cell precursors develop into mature T cells. In these organ cultures, as in the adult or neonatal beta ...

Virulent strains of Mycoplasma pneumoniae, PI-1428 and M129, were radiolabeled wtih [3H]palmitic acid or [3H]thymidine and examined for attachment to hamster tracheal organ cultures, tracheal outgrowth monolayers, human O-positive erythrocytes, and human WiDr carcinoma cell cultures. Although attachment to each cell substrate was readily detected, the WiDr cell culture monolayers provided the m...

Bronchial secretion forms a periciliary liquid layer and may also give rise to thicker secretion that moves on the tips of the cilia. This latter material as collected at bronchoscopy or coughed up as sputum is a viscoelastic substance behaving in a characteristic way over a wide range of shear rates. The anatomical basis of secretion is described and its control as established by organ culture.

Bronchial secretion forms a periciliary liquid layer and may also give rise to thicker secretion that moves on the tips of the cilia. This latter material as collected at bronchoscopy or coughed up as sputum is a viscoelastic substance behaving in a characteristic way over a wide range of shear rates. The anatomical basis of secretion is described and its control as established by organ culture.

Cell culture is an in vitro technique which cells, tissues, or organs (animal origin) are artificially grown with the support of artificial environment that encompasses medium, CO2 level, pH indicator, temperature keeping tissues alive and growing appropriately. Organ culture, Primary explant among them cell widely used for understanding growth, normal functions, identification growth factors, ...