This study was designed to investigate the effect of two surfactants, dodecyl benzyl sulfonate (DBS), sodium dodecyl sulfate (SDS), and of a domestic detergent (Y) on the AChE activity of the crustacean cladoceran Daphnia magna. All the chemicals significantly inhibit the activity of the enzyme, both in vitro and in vivo conditions. In vitro lowest observed effect concentration (LOEC) values ra...

The present study was conducted to evaluate the effects of an organophosphorous pesticide malathion on histo-architecture of liver and kidney of common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 8 days. The 96 h LC50 value of malathion for common carp was 15.24 mg/L. Several morphological changes were observed in the liver after exposure to the malathion. H...

Recombinant Escherichia coli systems expressing organophosphorous hydrolase (OPH) have been proposed for biotransformation of toxic organophosphate compounds. However, whole cell biocatalyst systems are critically disadvantaged due to substrate diffusion limitations. To enhance whole cell biocatalytic efficiency, we engineered E. coli, for the first time, to secrete metal ion cofactor-requiring...

Phosphatase activities (cell-bounded phosphatases "BP" and freely dissolved phosphatases "D P") in water samples from a natural lake "Laguna Grande" (Antequera, Málaga, Spain) amended with 50 microg/ml of selected insecticides, herbicides and fungicide captan were studied under laboratory controlled conditions (temperature and agitation). Our data show that dissolved alkaline phosphatase was th...

Introduction: Poisoning is an acute medical emergency. Organophosphorus (OP) poison, being easily available and relatively cheaper, mostly used by people for suicide. It a major health problem worldwide also cause of suicide in Nepal. The purpose this study was to assess the overall profile patients with poisoning. Methods: A Prospective hospital based descriptive cross-sectional conducted on o...