Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

Follicle culture systems have been developed so as to achieve in vitro fertilization of oocytes coming from immature follicles. The in vitro folliculogenesis methods would be especially useful in reproductive medicine to restore fertility in women having undergone ovarian cryopreservation. Several culture systems allowing in vitro growth of small follicles have been developed in mouse. These ha...

BACKGROUND Infertility due to accelerated loss of ovarian follicles/oocytes may occur through numerous mechanisms. As a result, posthumous human oocyte donation, banking and maturation protocols for research and fertility restoration are current interests in reproductive medicine. METHODS A computer-generated sample of Utah residents (n = 704) were surveyed regarding demographics, willingness...

The interaction of Streptococcus pneumoniae with human ciliated upper respiratory mucosa was studied in an agar-embedded organ culture of nasal turbinate tissue, which only exposed the intact epithelial surface and its secretion. The ciliary beat frequency, measured along the edge of the organ culture, was slowed by 13% in the presence of S. pneumoniae after 16 h (p less than 0.05) compared wit...

Organ culture specifically inhibits vasorelaxation to acute hypoxia and preferentially decreases specific voltage-dependent K(+) channel expression over other K(+) and Ca(2+) channel subtypes. To isolate further potential oxygen-sensing mechanisms correlated with altered gene expression, we performed differential display analysis on RNA isolated from control and cultured coronary arterial rings...

Growth-regulated oncogene A (GROA), a member of the chemokine superfamily, is commonly expressed in transformed cells and contributes to angiogenesis and tumorigenesis. Here, we report that increased GROA levels are detected in the plasma and ascites of ovarian cancer patients. Ovarian cancer cell lines in culture express and secrete GROA. However, when they are starved in serum-free medium, ov...

background: many health problems are related to lifestyle and dietary factors. since ancient times, food additives such as sulfites have been used to preserve foods. diverse effects of sulfites on multiple organs have been reported but its effect on female reproductive organ has not been fully elucidated. the aim of this study was to investigate the effects of sodium metabisulfite (smb) on ovar...

PURPOSE The aim of this study was to investigate the role of pericytes in regulating malignant ovarian cancer progression. EXPERIMENTAL DESIGN The pericyte mRNA signature was used to interrogate ovarian cancer patient datasets to determine its prognostic value for recurrence and mortality. Xenograft models of ovarian cancer were used to determine if co-injection with pericytes affected tumor ...

Vitamin D Receptor (VDR) is expressed in both animal and human ovarian tissue, however, the role of vitamin D in human ovarian steroidogenesis is unknown. Cultured human ovarian cells were incubated in tissue culture medium supplemented with appropriate substrates, with or without 50 pM-150 pM or 50 nM-150 nM of 1,25-(OH)2D3, and in the presence or absence of insulin. Progesterone, testosterone...