BACKGROUND The impact of prion proteins in the rules that dictate biological reproduction is still poorly understood. Likewise, the role of prnt gene, encoding the prion-like protein testis specific (Prt), in ram reproductive physiology remains largely unknown. In this study, we assessed the effect of Prt in ovine fertilization by using an anti-Prt antibody (APPA) in fertilization medium incuba...

Abstract The objective of this study was to use matrix-assisted laser desorption ionisation–time flight mass spectrometry (MALDI-TOF MS) for the identification ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank and cheese samples characterised by conventional phenotypic methods. A total 69 bacterial fu...

The mammalian oviduct is an anatomical part of the female reproductive tract, which plays several important roles in the events related to fertilization and embryo development. This review examines and compares several studies related to the proteomic and transcriptomic profile of the oviduct in different domestic animals. This information could be important for clarifying the role of oviductal...

background: successful embryo transfer (et) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. genetic ...

We have identified a CD8⁺CXCR5⁺ T cell that prevents the development of oviduct dilation following C. muridarum genital infection. Phenotypic studies show that CD8⁺CXCR5⁺ cells express markers of T regulatory cells (FoxP3, CD25, and GITR) but do not express a necessary component of cytotoxic cells (perforin). Cxcr5⁻/⁻ mice have significantly lower numbers of CD8⁺ cells and lack the CD8⁺CXCR5⁺ p...

The beta subunit of follicle stimulating hormone (FSHB) is expressed specifically in pituitary gonadotropes in vertebrates. Transgenic mouse studies have shown that enhancers in the proximal promoter between -172/-1 bp of the ovine FSHB gene are required for gonadotrope expression of ovine FSHB. These enhancers are associated with regulation by activins and gonadotropin releasing hormone (GnRH)...

A clone of the extracellular domain of the rat liver prolactin receptor was generated by the RNA-based polymerase chain reaction, and the NH2-terminal 210 amino acids were expressed in HeLa cells using a vaccinia virus/T7 hybrid expression system. The protein was isolated from serum-free culture medium directly by chromatography on an ovine prolactin affinity column and yielded approximately 1....

The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compar...

Objective: The study was conducted on quail in a specialized area of the laboratory histopathological disorders from September 6th to November 23rd, 2018. goal this research determine impact adding an alcohol extract Propolis ovarian and oviduct tissue ovulation fertility rates quail. method is as follows: Enter into eight groups with replicates within each group. Weight matched were housed 13 ...

Mammalian oviduct is the physiological site for sperm capacitation, gamete fertilization and early embryonic development. The secretory cells lining the lumen of the mammalian oviduct synthesize and secrete high molecular weight glycoprotein (OGP) in response to estrogen. The protein has been shown to interact with gametes and early embryo. Several key functions have been postulated particularl...