Corticosterone (CORT)-induced oxidative stress and neurotoxicity can cause neuronal dysfunction mental disorders. In the present study, we investigated effects mechanism of HP-20 resin fraction water extract Vaccinium bracteatum leaves (NET-D1602) its bioactive compound p-coumaric acid on cell damage in SH-SY5Y cells primary culture rat cortical cells. NET-D1602 significantly improved viability...

BACKGROUND Evidence exists that oxidative stress promotes the tyrosine phosphorylation of N-methyl-D-aspartate receptor (NMDAR) subunits during post-ischemic reperfusion of brain tissue. Increased tyrosine phosphorylation of NMDAR NR2A subunits has been reported to potentiate receptor function and exacerbate NMDAR-induced excitotoxicity. Though the effect of ischemia on tyrosine phosphorylation...

The objective of the present study was to obtain large amounts of submitochondrial fragments which would carry out oxidative phosphorylation with only a few substrates. It was the desire of the authors to have a system as free of other reaction sequences and with as little extraneous material as possible in order to study the mechanism of the reactions involved in oxidative phosphorylation. Sev...

Mitochondrial function is a key determinant of both excitability and viability of neurons. Here, we demonstrate seizure-dependent changes in mitochondrial oxidative phosphorylation in the epileptic rat hippocampus. The intense pathological neuronal activity in pilocarpine-treated rats exhibiting spontaneous seizures resulted in a selective decline of the activities of NADH-CoQ oxidoreductase (c...

The purple photosynthetic bacterium, Rhodospirillum rubrum, grows anaerobically only when illuminated with light of appropriate wave lengths. Extracts prepared from cells grown in this manner catalyze an anaerobic light-dependent phosphorylation of adenosine diphosphate to adenosine triphosphate (1). However, R. rubrum also can be grown heterotrophically in darkness if oxygen is provided (2). U...

Protein kinase D (PKD) participates in activation of the transcription factor NFB (nuclear factor B) in cells exposed to oxidative stress, leading to increased cellular survival. We previously demonstrated that phosphorylation of PKD at Tyr463 in the PH (pleckstrin homology) domain is mediated by the Src-Abl pathway and that it is necessary for PKD activation and subsequent NFB induction. Here ...

BACKGROUND Oxidative stress has been suggested as a mechanism underlying skin aging, as it triggers apoptosis in various cell types, including fibroblasts, which play important roles in the preservation of healthy, youthful skin. Catechins, which are antioxidants contained in green tea, exert various actions such as anti-inflammatory, anti-bacterial, and anti-cancer actions. In this study, we i...

Oxidative stress is a major pathophysiological mediator of degenerative processes in many neurodegenerative diseases including Parkinson's disease (PD). Aberrant cell signaling governed by protein phosphorylation has been linked to oxidative damage of dopaminergic neurons in PD. Although several studies have associated activation of certain protein kinases with apoptotic cell death in PD, very ...

Abstract The inflammasome comprised of an innate immune receptor/sensor, pro-caspase-1, and a common adaptor molecule, ASC (apoptotic speck-containing protein with CARD) initiates defense inflammatory response by activating caspase-1 pyroptotic cell death in myeloid cells. Consistent their pro-inflammatory function, caspase-1, NLRP3 were known to exacerbate autoimmunity during experimental auto...

1. The ability of 4,6-dinitrocresol and eight other substituted phenols to stimulate oxygen uptake and inhibit phosphorylation by a cell-free particulate system from unfertilized Arbacia eggs has been determined. Five of those agents can produce both stimulation of oxygen consumption and inhibition of phosphorylation; one inhibits both oxygen consumption and phosphorylation; and two have no eff...